Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis.
Liu MS, Huang TK, Wang YC, Wang SC, Wu CH, Kuo CH, Lai EM
New Phytol. 2025 Nov 30. doi: 10.1111/nph.70795.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234350 | EML6428_ pRL662-eGFP | Expressed eGFP(S65T) in agrobacteria for tracing in planta |
| 234351 | EML6442_p35S-mCherry2-SV40 | Expressed mCherry2 florescent proteins in plant cells for tracing transformation events |
| 234352 | EML8357_pA3A-CBECas9 | A base editor derived by egg-cell specific promoter |
| 234353 | EML8359_pA3A-CBECas9-ALSpro197 | Base editor targets to ALS Pro197 locus |
| 237864 | EML8543_pEML41003 | A suicide vector for Agrobacterium with kanamycin resistance. |
| 237865 | EML8552_pEML41003-tufApst4-15 | A suicide vector for replacing 4th to 15th amino acids of tufA |
| 237866 | EML8553_ pEML41003-tufBpst4-15 | A suicide vector for replacing 4th to 15th amino acids of tufB |