Directed evolution of LaccID for cell surface proximity labeling and electron microscopy.
Lee SY, Roh H, Gonzalez-Perez D, Mackey MR, Hoces D, McLaughlin CN, Lin C, Adams SR, Nguyen K, Kim KY, Luginbuhl DJ, Luo L, Udeshi ND, Carr SA, Hernandez-Lopez RA, Ellisman MH, Alcalde M, Ting AY
Nat Chem Biol. 2025 Aug 1. doi: 10.1038/s41589-025-01973-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234046 | pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay | expresses LaccID on the mammalian cell surface |
| 234047 | pTRE3G_SS-HA-V5-LaccID-CD4 TM_Puro | expresses LaccID on the mammalian cell surface, dox-inducible lentiviral vector |
| 234048 | pMND_IgKss-V5-HA-LaccID(codon opt)-CD4 TM_pHR | expresses LaccID on the T cell surface, lentiviral vector |
| 234049 | Aga2p-TEVcs-LaccID-myc_pCTCON2 | expresses LaccID on the yeast surface |
| 234050 | 10xUAS-wgSS-HA-V5-LaccID-CD2 (fly optimized, repeat fixed) | expresses LaccID on the surface of fly neurons |
| 234051 | pTRE3G_ManII-LaccID-V5_Puro | expresses LaccID in the Golgi apparatus, dox-inducible lentiviral vector |
| 234652 | pJRoC30-LaccID | Expresses LaccID evolved variant in yeast |
| 234653 | pJRoC30-LacAnc100 | Expresses LacAnc100 variant in yeast |