Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization.

Zhao C, Cao Y, Ibrahim N, Wang Y, Martemyanov KA
Nat Commun. 2025 Apr 22;16(1):3768. doi: 10.1038/s41467-025-58945-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
234724	pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori	All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
234737	pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori	All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.