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Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing.

Zhao Y, Huang Z, Zhou X, Teng W, Liu Z, Wang W, Tang S, Liu Y, Liu J, Wang W, Chai L, Zhang N, Guo W, Liu J, Ni Z, Sun Q, Wang Y, Zong Y
Nat Plants. 2025 Feb;11(2):191-205. doi: 10.1038/s41477-024-01898-3. Epub 2025 Jan 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
235161pB–DualPEFor plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons
236416pH-2×35S-DualPEFor plant prime editing including large DNA fragment editing in Nicotiana benthamiana or other dicotyledons
236417pH-EF1α-DualPEFor plant prime editing including large DNA fragment editing in Solanum lycopersicum or other dicotyledons

Antibodies from Article