Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS.
Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig T, Dolken L, Lomonte P, Fachinetti D, Manel N
Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235527 | pLKO.1-puro-USP7-sh1 | shRNA against human USP7 |
| 235528 | pLKO.1-puro-USP7-sh5 | shRNA against human USP7 |
| 235529 | pLentiCRISPRv2 Neo-MORC3_gRNA_1 | gRNA against human MORC3 |
| 235530 | pLentiCRISPRv2 Neo-MORC3_gRNA_3 | gRNA against human MORC3 |
| 235531 | pLentiCRISPRv2_cGAS_gRNA_3 | gRNA against human cGAS |
| 235532 | pLentiCRISPRv2_IFNB1_gRNA_1 | gRNA against human IFNB1 |
| 235533 | pLentiCRISPRv2_IFNB1_gRNA_3 | gRNA against human IFNB1 |
| 235534 | pTRIP-CMV | To express GFP |
| 235535 | pTRIP-SFFV-tagBFP-2A-ICP0 | To express tagBFP-2A-ICP0 |
| 235536 | pTRIP-SFFV-tagBFP-2A-coICP0 | To express tagBFP-2A-coICP0 (co = codon-optimized) |
| 235537 | pTRIP-SFFV-tagRFP657-2A | To express tagRFP657-2A |
| 235538 | pTRIP-SFFV-tagRFP657-2A-coICP0 | To express tagRFP657-2A-coICP0 (co = codon-optimized) |
| 235539 | pTRIP-SFFV-tagBFP-2A-ICP0-FxE | To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149) |
| 235540 | pTRIP-SFFV-tagBFP-2A-coICP0-∆USP7 | To express tagBFP-2A-coICP0-∆USP7 (ICP0 ∆618-633, co = codon-optimized) |
| 235541 | pTRIP-SFFV-tagBFP-2A-IE1 | To express tagBFP-2A-IE1 |
| 235542 | pTRIP-SFFV-tagBFP-2A-FLAG-coK8 | To express tagBFP-2A-FLAG-coK8 (co = codon-optimized) |
| 235544 | pTRIP-SFFV-tagBFP-2A-FLAG-coORF63 | To express tagBFP-2A-FLAG-coORF8 (co = codon-optimized) |
| 235545 | pEasiLV-MCS-coICP0 | To express ICP0 (doxycycline inducible) |
| 235546 | pEasiLV-MCS-coICP0fxe | To express ICP0-FXE (ICP0 ∆106-149, doxycycline inducible) |
| 235547 | pTRIP-SFFV | To express GFP |
| 235548 | pTRIP-SFFV-EGFP-FLAG-UL36USP (C40) | To express GFP-FLAG-UL36USP (C40) - region 25-520 of UL36 |
| 235549 | pTRIP-SFFV-EGFP-FLAG-UL36USP (C65) | To express GFP-FLAG-UL36USP (C65) - region 1-520 of UL36 |