Tom Ellis Lab: Ellis genetic toolkit
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235329 | STK002_pSTK-1A-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235331 | STK004_pSTK-1C-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235332 | STK005_pSTK-1D-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235333 | STK013_pSTK-1A-lacZ | Destination plasmid containing a LacZ dropout |
| 235336 | STK016_pSTK-1D-lacZ | Destination plasmid containing a LacZ dropout |
| 235338 | STK202_pSTK-EXP-1-LacZ | Shuttle vector E. coli - B. subtilis. Produced from pNW33N adapted for STK |
| 235343 | STK083_pSTK-1A-5'-lacA | 1000 bp size. 5' and 3' end are referenced to gene orientation |
| 235344 | STK084_pSTK-1D-3'-lacA | 1000 bp size. 5' and 3' end are referenced to gene orientation |
| 235345 | STK085_pSTK-1A-5'-sacA | 1000 bp size. 5' and 3' end are referenced to gene orientation |
| 235346 | STK086_pSTK-1D-3'-sacA | 1000 bp size. 5' and 3' end are referenced to gene orientation |
| 235347 | STK087_pSTK-1A-5'-xynP | 1001 bp size. 5' and 3' end are referenced to gene orientation |
| 235348 | STK088_pSTK-1D-3'-xynP | 1002 bp size. 5' and 3' end are referenced to gene orientation |
| 235349 | STK093_pSTK-1A-Spacer | Cloning spacer |
| 235350 | STK094_pSTK-1B-Spacer | Cloning spacer |
| 235351 | STK095_pSTK-1C-Spacer | Cloning spacer |
| 235352 | STK096_pSTK-1D-Spacer | Cloning spacer |
| 235353 | STK001_pSTK-0-sfGFP | Entry-level plasmid containing a sfGFP dropout cassette |
| 235354 | STK006_pSTK-2A-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235355 | STK007_pSTK-2B-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235356 | STK008_pSTK-2C-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235357 | STK009_pSTK-2D-sfGFP | Destination plasmid containing a sfGFP dropout |
| 235359 | STK018_pSTK-2B-lacZ | Destination plasmid containing a LacZ dropout |
| 235364 | STK026_pSTK-0A-Peps | Biofilm specific promoter in B. subtilis |
| 235365 | STK027_pSTK-0A-Phag | Flagellin promoter in B. subtilis |
| 235366 | STK028_pSTK-0A-Pveg (BBa_K823003) | Med-strong constitutive promoter |
| 235367 | STK029_pSTK-0A-PlepA | Low expression constitutive promoter in B. subtilis |
| 235368 | STK030_pSTK-0A-PliaG | Constitutive promoter in B. subtilis |
| 235370 | STK033_pSTK-0A-PsspB | Sporulation promoter. Expression after the initiation of sporulation in B. subtilis. |
| 235371 | STK034_pSTK-0A-Phyperspank | Medium constitutive promoter. Note: Requires LacI to be inducible with IPTG. |
| 235372 | STK116_pSTK-0A-BBa_K090503 | Low expression constitutive promoter in B. subtilis |
| 235374 | STK035_pSTK-0AB-PtagC+RBS | Medium constitutive promoter. Note: Requires LacI to be inducible with IPTG. |
| 235375 | STK042_pSTK-0B-3P promoter | Very strong constitutive promoter in B. subtilis |
| 235376 | STK160_pSTK-0A-MtlR-Pmtl | Mannitol inducible promoter |
| 235377 | STK161_pSTK-0A-XylR-Pxyl | Xylose inducible promoter |
| 235378 | STK162_pSTK-0A-GlyR-Pgly | Glycerol inducible promoter |
| 235379 | STK163_pSTK-0A-ManR-Pman | Mannose inducible promoter |
| 235380 | STK046_pSTK-0B-TM_RBS2 | Weak RBS in B. subtilis. Do not confuse with RBS2 (strong) |
| 235381 | STK048_pSTK-0B-TM_RBS4 | Weak RBS in B. subtilis |
| 235382 | STK043_pSTK-0B-PET_RBS | Medium RBS in B. subtilis. Strong RBS in E. coli |
| 235383 | STK204_pSTK0-B-RBS0 | Very strong RBS in B. subtilis |
| 235384 | STK205_pSTK0-B-RBS2 | Strong RBS |
| 235385 | STK190_pSTK0B-RiboJ-RBS0 | Very strong RBS in B. subtilis. RiboJ is a self cutting 5' region of the mRNA to avoid interactions with the RBS. |
| 235386 | STK207_pSTK0B-RS-TT-RS-RiboJ-RBS0 | Very strong RBS in B. subtilis with an E. coli Expression Blocking Device (EEBD). Impedes expression in E. coli and recombines in B. subtilis to permit expression. |
| 235387 | STK052_pSTK-0C-mEYFP | Yellow fluorescent protein |
| 235388 | STK053_pSTK-0C-GFPmut3b | Green fluorescence protein |
| 235389 | STK054_pSTK-0C-mScarlet-I | Brilliant red fluorescence protein |
| 235390 | STK055_pSTK-0C-eCFP codon optimized BS | Cyan fluorescence protein codon optimized for B. subtilis |
| 235391 | STK125_pSTK-0C-mCherry | Pink fluoescent protein |
| 235392 | STK127_pSTK0C-mOrange | Orange fluorescent protein |
| 235393 | STK138_pSTK-0C-mRFP1 | Violet fluorescent protein |
| 235394 | STK060_pSTK-C0x-TasA secretion tag | Biofilm specific secretion tag in B. subtilis |
| 235395 | STK230_pSTK-0Cx-SacB secretion tag | Secretion tag |
| 235396 | STK284_pSTK-0Cy-GanA-Galactosidase | Secretion tag |
| 235397 | STK330_pSTK-0Cx-AmyQ secretion tag | Secretion tag |
| 235398 | STK331_pSTK-0Cx-Csn secretion tag | Secretion tag |
| 235400 | STK333_pSTK-0Cx-YlqB secretion tag | Secretion tag |
| 235401 | STK381_pSTK-0Cx-AprSecTag | Secretion tag |
| 235403 | STK062_pSTK-0Cz-GFPmut3b | Green fluorescence protein with stop codon. |
| 235404 | STK063_pSTK-0Cz-His6x | 6xHis tag for protein purification |
| 235405 | STK064_pSTK-0Cz-FLAG | Flag tag for Western blot |
| 235406 | STK065_pSTK-0Cz-StrepII | StrepII tag for protein purification |
| 235407 | STK066_pSTK-0Cz-SsrA | SsrA tag for tuning protein degradation (AANDENYALAA) |
| 235408 | STK067_pSTK-0Cz-SpyTag | SpyTag for bioconjugation. Under patent WO2020188350A1 |
| 235409 | STK068_pSTK-0Cz-SpyCatcher | SpyCatcher for bioconjugation. Under patent WO2020188350A1 |
| 235410 | STK069_pSTK-0D-B0012 | Strong terminator in B. subtilis. Weak transcriptional terminator in E. coli |
| 235413 | STK078_pSTK-0D-L3S3P21b | Very strong terminator in B. subtilis. Strong synthetic transcriptional terminator in E. coli. Recombinant resistant. Modified to remove BsaI site (Position 49G to 49T). |
| 235414 | STK080_pSTK-0D-bkdB Cold double terminator | Very strong terminator in B. subtilis. Transcriptional terminator and 3'-mRNA stabilizer from bkdB gene of B. subtilis. Positive impact in expression at 18C. Contain STOP codons in both directions |
| 235415 | STK089_pSTK-0A-Spacer | Cloning spacer |
| 235416 | STK090_pSTK-0B-Spacer | Cloning spacer |
| 235419 | STK244_pSTK-0Cx-Spacer | Cloning spacer |
| 235420 | STK245_pSTK-0Cz-Spacer | Cloning spacer |
| 235421 | STK153_pSTK-2A-SpacerL2A | Cloning spacer |
| 235422 | STK154_pSTK-2B-SpacerL2B | Cloning spacer |
| 235423 | STK155_pSTK-2C-SpacerL2C | Cloning spacer |
| 235425 | STK168_pSTK-0A-PGEO50 | Consituive promoter for Geobacillus and Parageobacillus |
| 235426 | STK169_pSTK-0A-PGEO56 | Consituive promoter for Geobacillus and Parageobacillus |
| 235427 | STK170_pSTK-0A-PGEO64 | Consituive promoter for Geobacillus and Parageobacillus |
| 235428 | STK171_pSTK-0A-PGEO65 | Consituive promoter for Geobacillus and Parageobacillus |
| 235429 | STK172_pSTK-0A-PGEO66 | Consituive promoter for Geobacillus and Parageobacillus |
| 235430 | STK173_pSTK-0A-PGEO67 | Consituive promoter for Geobacillus and Parageobacillus |
| 235431 | STK174_pSTK-0A-PGEO68 | Consituive promoter for Geobacillus and Parageobacillus |
| 235432 | STK175_pSTK-0A-PGEO69 | Consituive promoter for Geobacillus and Parageobacillus |
| 235433 | STK176_pSTK-0A-PGEO73 | Consituive promoter for Geobacillus and Parageobacillus |
| 235434 | STK177_pSTK-0A-PGEO74 | Consituive promoter for Geobacillus and Parageobacillus |
| 235435 | STK178_pSTK-0A-PGEO78 | Consituive promoter for Geobacillus and Parageobacillus |
| 235436 | STK179_pSTK-0A-PGEO79 | Consituive promoter for Geobacillus and Parageobacillus |
| 235438 | STK181_pSTK-0B-PGEO79RBS | Strong native RBS from Geobacillus with upstream RiboJ |
| 235440 | STK183_pSTK-0D-G.gluc_654 | Medium termination efficiency in P. thermoglucosidasius |
| 235441 | STK184_pSTK-0D-G.sub_274 | Weak termination efficiency in P. thermoglucosidasius |