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Tobias Erb Lab: Advancing chloroplast synthetic biology through high-throughput plastome engineering of Chlamydomonas reinhardtii

Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J, Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund F, Mackinder LCM, Erb TJ
bioRxiv 2024.05.08.593163 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
235784pME_Cp_0_1_001_psbH_5'Hom_Cr5' Homology psbH in the M1 position used for genome integration at the psbH locus
235785pME_Cp_0_1_004_psbA_5'Hom_Cr5' Homology psbA in the M1 position used for genome integration at the psbA locus
235787pME_Cp_0_1_007_petB_5'Hom_cr5' Homology petB in the M1 position used for genome integration at the petB locus
235789pME_G_0_1_005_5C1SF5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly
235790pME_G_0_1_008_5C2SF5' Connector 2 Short Forward in the M1 position used to dictate position for lvl2 assembly
235791pME_G_0_1_011_5C3SF5' Connector 3 Short Forward in the M1 position used to dictate position for lvl2 assembly
235792pME_G_0_1_022_5C4SF5' Connector 4 Short Forward in the M1 position used to dictate position for lvl2 assembly
235793pME_G_0_1_014_5C5SF5' Connector 5 Short Forward in the M1 position used to dictate position for lvl2 assembly
235795pME_G_0_1_017_5C7SF5' Connector 7 Short Forward in the M1 position used to dictate position for lvl2 assembly
235796pME_G_0_1_006_5C1SR5' Connector 1 Short Reverse in the M1 position used to dictate position for lvl2 assembly
235797pME_G_0_1_009_5C2SR5' Connector 2 Short Reverse in the M1 position used to dictate position for lvl2 assembly
235800pME_G_0_1_019_5C7SR5' Connector 7 Short Reverse in the M1 position used to dictate position for lvl2 assembly
235801pME_G_0_1-2_001_OC5'15' Operon Connector 1 in the M1 position used to construct operons
235802pME_G_0_1-2_002_OC5'25' Operon Connector 2 in the M1 position used to construct operons
235803pME_G_0_1-2_003_OC5'35' Operon Connector 3 in the M1 position used to construct operons
235805pME_G_0_1-2_005_OC5'55' Operon Connector 5 in the M1 position used to construct operons
235806pME_G_0_1-2_006_OC5'65' Operon Connector 6 in the M1 position used to construct operons
235807pME_G_0_1-2_007_OC5'75' Operon Connector 7 in the M1 position used to construct operons
235808pME_G_0_2_001_Promoter_placeholderEncodes a sfGFP cassette in the promoter position used for placeholder cloning
235809pME_B_0_2_020_PdummyPromoter dummy sequence used as a baseline for promoter characterization
235810pME_Cp_0_2_001_rrn16_prom_Crrrn16 promoter long Chlamydomonas reinhardtii
235811pME_Cp_0_2_003_psaA2_prom_CrpsaA2 promoter Chlamydomonas reinhardtii
235812pME_Cp_0_2_004_rrn16_prom_short_Crrrn16 promoter short Chlamydomonas reinhardtii
235831pME_B_0_3_009_5'UTR_dummy5'UTR dummy sequence used as a baseline in 5'UTR characterization
235832pME_Cp_0_3_002_atpA_5'UTR_CratpA 5'UTR Chlamydomonas reinhardtii
235833pME_Cp_0_3_005_petB_5'UTR_CrpetB 5'UTR Chlamydomonas reinhardtii
235834pME_Cp_0_3_006_psaA_5'UTR_CrpsaA 5'UTR Chlamydomonas reinhardtii
235835pME_Cp_0_3_007_psbA_5'UTR_CrpsbA 5'UTR Chlamydomonas reinhardtii
235836pME_Cp_0_3_008_psbE_5'UTR_CrpsbE 5'UTR Chlamydomonas reinhardtii
235838pME_Cp_0_3_010_psbK_5'UTR_CrpsbK 5'UTR Chlamydomonas reinhardtii
235839pME_Cp_0_3_011_petA_5'UTR_CrpetA 5'UTR Chlamydomonas reinhardtii
235843pME_Cp_0_3_018_psaC_5'UTR_CrpsaC 5'UTR Chlamydomonas reinhardtii
235844pME_Cp_0_3_019_rbcL_5'UTR_CrrbcL 5'UTR Chlamydomonas reinhardtii
235845pME_Cp_0_3_020_rpoC1_5'UTR_CrrpoC1 5'UTR Chlamydomonas reinhardtii
235846pME_Cp_0_3_023_tufA_5'UTR_CrtufA 5'UTR Chlamydomonas reinhardtii
235847pME_Cp_0_3_024_clpP_5'UTR_CrclpP 5'UTR Chlamydomonas reinhardtii
235848pME_Cp_0_3_026_rpl16_5'UTR_Crrpl16 5'UTR Chlamydomonas reinhardtii
235849pME_Cp_0_3_030_atpH_5'UTR_CratpH 5'UTR Chlamydomonas reinhardtii
235852pME_Cp_0_3_061_gene10_5'UTR_T7gene10 5'UTR T7
235853pME_G_0_3a_001_PlaceholderEncodes a sfGFP cassette in the 3a position
235854pME_Cp_0_3a_001_gene10_5'UTR_T7gene10 5'UTR T7 3a
235855pME_Cp_0_3a_002_petA_5'UTR_CrpetA 5'UTR Chlamydomonas reinhardtii
235856pME_Cp_0_3a_003_psaA_5'UTR_CrpsaA 5'UTR Chlamydomonas reinhardtii
235857pME_Cp_0_3a_004_psbE_5'UTR_CrpsbE 5'UTR Chlamydomonas reinhardtii
235858pME_Cp_0_3a_005_rbcL_5'UTR_CrrbcL 5'UTR Chlamydomonas reinhardtii
235859pME_Cp_0_3IEE_001_atpE-ycf12_IEE_CratpE-ycf12 IEE Chlamydomonas reinhardtii
235862pME_Cp_0_3IEE_007_psbB-psbT_IEE_CrpsbB-psbT IEE Chlamydomonas reinhardtii
235863pME_Cp_0_3IEE_008_psbE-rps9_IEE_CrpsbE-rps9 IEE Chlamydomonas reinhardtii
235864pME_Cp_0_3IEE_013_rpl2-rps19_IEE_Crrpl2-rps19 IEE Chlamydomonas reinhardtii
235865pME_Cp_0_3IEE_015_rpl23-rpl2_IEE_Crrpl23-rpl2 IEE Chlamydomonas reinhardtii
235867pME_Cp_0_3IEE_021_ycf3-rps18_IEE_Crycf3-rps18 IEE Chlamydomonas reinhardtii
235868pME_Cp_0_3IEE_022_ycf4-ycf3_IEE_Crycf4-ycf3 IEE Chlamydomonas reinhardtii
235871pME_Cp_0_2-3_001_psbD_Promoter+5'UTR_CrpsbD promoter + 5'UTR Chlamydomonas reinhardtii
235872pME_Cp_0_2-3_002_rbcL_Promoter+5'UTR_CrrbcL promoter + 5'UTR Chlamydomonas reinhardtii
235874pME_Cp_0_2-3a_002_psbD_Promoter+5'UTR_CrpsbD promoter + 5'UTR Chlamydomonas reinhardtii 2-3a
235875pME_Cp_0_2-3a_003_rbcL_Promoter+5'UTR_CrrbcL promoter + 5'UTR Chlamydomonas reinhardtii 2-3a
235878pME_Cp_0_3b_025_Hibit-tagHiBiT N-tag
235879pME_Cp_0_3b_026_Flag-tagFLAG N-tag
235880pME_Cp_0_3b_027_HA-tagHA N-tag
235881pME_Cp_0_3b_028_MYC-tagMYC N-tag
235882pME_Cp_0_3b_029_mScarlet-ImScarlet-I N-tag
235883pME_Cp_0_3b_030_mCeruleanmCerulean N-tag
235884pME_Cp_0_3b_031_mVenusmVenus N-tag
235885pME_Cp_0_5a_003_Hibit-tagHiBiT C-tag
235886pME_Cp_0_5a_004_Flag-tagFLAG C-tag
235887pME_Cp_0_5a_005_HA-tagHA C-tag
235890pME_Cp_0_5a_008_mCeruleanmCerulean C-tag
235891pME_Cp_0_5a_009_mVenusmVenus C-tag
235892pME_G_0_4_001_CDS_PlaceholderEncodes a sfGFP cassette in the CDS position used for placeholder cloning
235893pME_Cp_0_4_001_NanolucNanoluc luciferase reporter gene, codon optimized for Chlamydomonas reinhardtii
235894pME_Cp_0_4_007_mScarlet-ImScarlet-I reporter gene, codon optimized for Chlamydomonas reinhardtii
235895pME_Cp_0_4_008_tdTomatotdTomato reporter gene, codon optimized for Chlamydomonas reinhardtii
235898pME_Cp_0_4_022_aac6-aph2-markeraac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii
235900pME_Cp_0_4_024_CrCD-markerCytosine deaminase selection gene, codon optimized for Chlamydomonas reinhardtii
235901pME_Cp_0_4_026_Arg9-markerN-acetyl ornithine aminotransferase selection gene, codon optimized for Chlamydomonas reinhardtii
235904pME_Cp_0_4_035_aphA6-KanAminoglycoside-3'-phosphotransferase selection gene, codon optimized for Chlamydomonas reinhardtii
235905pME_G_0_5_001_3'UTR_PlaceholderEncodes a sfGFP cassette in the 3'UTR position used for placeholder cloning
235906pME_B_0_5_012_3'UTR_dummy3'UTR dummy sequence used as a baseline in 3'UTR characterization
235907pME_Cp_0_5_001_psbA_3'UTR_CrpsbA 3'UTR Chlamydomonas reinhardtii
235908pME_Cp_0_5_005_rrnB_3'UTR_CrrrnB 3'UTR Chlamydomonas reinhardtii
235909pME_Cp_0_5_006_psbA_3'UTR_NtpsbA 3'UTR Nicotiana tabacum
235910pME_Cp_0_5_007_atpA_3'UTR_CratpA 3'UTR Chlamydomonas reinhardtii
235912pME_Cp_0_5_010_atpB_3'UTR_CratpB 3'UTR Chlamydomonas reinhardtii
235913pME_Cp_0_5_013_ftsH_3'UTR_CrftsH 3'UTR Chlamydomonas reinhardtii
235915pME_Cp_0_5_017_petD_3'UTR_CrpetD 3'UTR Chlamydomonas reinhardtii
235918pME_Cp_0_5_021_psbA_3'UTR_CrpsbA 3'UTR Chlamydomonas reinhardtii
235919pME_Cp_0_5_022_psbC_3'UTR_CrpsbC 3'UTR Chlamydomonas reinhardtii
235920pME_Cp_0_5_026_rbcL_3'UTR_CrrbcL 3'UTR Chlamydomonas reinhardtii
235922pME_Cp_0_5_032_rps2_3'UTR_Crrps2 3'UTR Chlamydomonas reinhardtii
235923pME_Cp_0_5_035_rps8_3'UTR_Crrps8 3'UTR Chlamydomonas reinhardtii
235924pME_Cp_0_5_036_rps11_3'UTR_Crrps11 3'UTR Chlamydomonas reinhardtii
235925pME_Cp_0_5_038_rps19_3'UTR_Crrps19 3'UTR Chlamydomonas reinhardtii
235926pME_Cp_0_5_039_tufA_3'UTR_CrtufA 3'UTR Chlamydomonas reinhardtii
235927pME_Cp_0_5_041_ycf12_3'UTR_Crycf12 3'UTR Chlamydomonas reinhardtii
235928pME_Cp_0_5_042_BMV_3'UTR_BvBMV 3'UTR Brome Mosaic Virus
235929pME_Cp_0_5_043_rbcL_3'UTR_NtrbcL 3'UTR Nicotiana tabacum
235930pME_Cp_0_5_045_rpoA_3'UTR_CrrpoA 3'UTR Chlamydomonas reinhardtii
235934pME_Cp_0_5b_001_psbA_3'UTR_CrpsbA 3'UTR Chlamydomonas reinhardtii
235935pME_Cp_0_5b_002_atpB_3'UTR_CratpB 3'UTR Chlamydomonas reinhardtii
235936pME_Cp_0_5b_003_petL_3'UTR_CrpetL 3'UTR Chlamydomonas reinhardtii
235937pME_Cp_0_5b_005_TMV_3'UTR_TvTMV 3'UTR Tobacco Mosaic Virus
235938pME_Cp_0_5b_006_ftsH_3'UTR_CrftsH 3'UTR Chlamydomonas reinhardtii
235940pME_Cp_0_6_006_psbH_5'Hom_rev_Cr5' Homology psbH reversee in the M2 position used for genome integration at the psbH locus
235942pME_Cp_0_6_009_rbcL_3'Hom_Cr3' Homology rbcL in the M2 position used for genome integration at the rbcL locus
235943pME_Cp_0_6_013_petB_3'Hom_Cr3' Homology petB in the M2 position used for genome integration at the petB locus
235944pME_Cp_0_6_019_psbD_3'Hom_Cr3' Homology psbD in the M2 position used for genome integration at the psbD locus
235946pME_Cp_0_6_021_petB_5'Hom_rev_Cr5'Homology petB reverse in the M2 position used for genome integration at the petB locus
235949pME_G_0_6_021_3C1SF3' Connector 1 Short Forward in the M2 position used to dictate position for lvl2 assembly
235950pME_G_0_6_022_3C2SF3' Connector 2 Short Forward in the M2 position used to dictate position for lvl2 assembly
235951pME_G_0_6_023_3C3SF3' Connector 3 Short Forward in the M2 position used to dictate position for lvl2 assembly
235952pME_G_0_6_024_3C4SF3' Connector 4 Short Forward in the M2 position used to dictate position for lvl2 assembly
235953pME_G_0_6_025_3C5SF3' Connector 5 Short Forward in the M2 position used to dictate position for lvl2 assembly
235955pME_G_0_6_027_3C7SF3' Connector 7 Short Forward in the M2 position used to dictate position for lvl2 assembly
235956pME_G_0_6_004_3C1LR3' Connector 1 Long Reverse in the M2 position used to dictate position for lvl2 assembly
235957pME_G_0_6_006_3C1SR3' Connector 1 Short Reverse in the M2 position used to dictate position for lvl2 assembly
235958pME_G_0_6_009_3C2SR3' Connector 2 Short Reverse in the M2 position used to dictate position for lvl2 assembly
235959pME_G_0_6_012_3C3SR3' Connector 3 Short Reverse in the M2 position used to dictate position for lvl2 assembly
235960pME_G_0_6_015_3C5SR3' Connector 5 Short Reverse in the M2 position used to dictate position for lvl2 assembly
235961pME_G_0_6_019_3C7SR3' Connector 7 Short Reverse in the M2 position used to dictate position for lvl2 assembly
235962pME_G_0_5-6_006_OC3'13' Operon Connector 1 in the M2 position used to construct operons
235963pME_G_0_5-6_007_OC3'23' Operon Connector 2 in the M2 position used to construct operons
235964pME_G_0_5-6_008_OC3'33' Operon Connector 3 in the M2 position used to construct operons
235965pME_G_0_5-6_009_OC3'43' Operon Connector 4 in the M2 position used to construct operons
235966pME_G_0_5-6_010_OC3'53' Operon Connector 5 in the M2 position used to construct operons
235967pME_G_0_5-6_011_OC3'63' Operon Connector 6 in the M2 position used to construct operons
235968pME_G_0_5-6_012_OC3'73' Operon Connector 7 in the M2 position used to construct operons
235970pME_Cp_0_7-8_004_Amp/ColEI_sfGFPAmpicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly
235973pME_Cp_UAV_sfGFPUniversal acceptor vector with sfGFP placeholder for lvl0 assembly
235975pME_G_E_007_UAV_sfGFP_PaqCIUniversal acceptor vector with sfGFP placeholder PaqCI compatible for lvl0 assembly

Antibodies from Article