Tobias Erb Lab: Advancing chloroplast synthetic biology through high-throughput plastome engineering of Chlamydomonas reinhardtii
Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J, Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund F, Mackinder LCM, Erb TJ
bioRxiv 2024.05.08.593163
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235784 | pME_Cp_0_1_001_psbH_5'Hom_Cr | 5' Homology psbH in the M1 position used for genome integration at the psbH locus |
| 235785 | pME_Cp_0_1_004_psbA_5'Hom_Cr | 5' Homology psbA in the M1 position used for genome integration at the psbA locus |
| 235786 | pME_Cp_0_1_005_rbcL_5'Hom_Cr | 5' Homology rbcL in the M1 position used for genome integration at the rbcL locus |
| 235787 | pME_Cp_0_1_007_petB_5'Hom_cr | 5' Homology petB in the M1 position used for genome integration at the petB locus |
| 235788 | pME_Cp_0_1_010_psbD_5'Hom_Cr | 5' Homology psbD in the M1 position used for genome integration at the psbD locus |
| 235789 | pME_G_0_1_005_5C1SF | 5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235790 | pME_G_0_1_008_5C2SF | 5' Connector 2 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235791 | pME_G_0_1_011_5C3SF | 5' Connector 3 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235792 | pME_G_0_1_022_5C4SF | 5' Connector 4 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235793 | pME_G_0_1_014_5C5SF | 5' Connector 5 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235794 | pME_G_0_1_023_5C6SF | 5' Connector 6 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235795 | pME_G_0_1_017_5C7SF | 5' Connector 7 Short Forward in the M1 position used to dictate position for lvl2 assembly |
| 235796 | pME_G_0_1_006_5C1SR | 5' Connector 1 Short Reverse in the M1 position used to dictate position for lvl2 assembly |
| 235797 | pME_G_0_1_009_5C2SR | 5' Connector 2 Short Reverse in the M1 position used to dictate position for lvl2 assembly |
| 235798 | pME_G_0_1_012_5C3SR | 5' Connector 3 Short Reverse in the M1 position used to dictate position for lvl2 assembly |
| 235799 | pME_G_0_1_015_5C5SR | 5' Connector 6 Short Reverse in the M1 position used to dictate position for lvl2 assembly |
| 235800 | pME_G_0_1_019_5C7SR | 5' Connector 7 Short Reverse in the M1 position used to dictate position for lvl2 assembly |
| 235801 | pME_G_0_1-2_001_OC5'1 | 5' Operon Connector 1 in the M1 position used to construct operons |
| 235802 | pME_G_0_1-2_002_OC5'2 | 5' Operon Connector 2 in the M1 position used to construct operons |
| 235803 | pME_G_0_1-2_003_OC5'3 | 5' Operon Connector 3 in the M1 position used to construct operons |
| 235804 | pME_G_0_1-2_004_OC5'4 | 5' Operon Connector 4 in the M1 position used to construct operons |
| 235805 | pME_G_0_1-2_005_OC5'5 | 5' Operon Connector 5 in the M1 position used to construct operons |
| 235806 | pME_G_0_1-2_006_OC5'6 | 5' Operon Connector 6 in the M1 position used to construct operons |
| 235807 | pME_G_0_1-2_007_OC5'7 | 5' Operon Connector 7 in the M1 position used to construct operons |
| 235808 | pME_G_0_2_001_Promoter_placeholder | Encodes a sfGFP cassette in the promoter position used for placeholder cloning |
| 235809 | pME_B_0_2_020_Pdummy | Promoter dummy sequence used as a baseline for promoter characterization |
| 235810 | pME_Cp_0_2_001_rrn16_prom_Cr | rrn16 promoter long Chlamydomonas reinhardtii |
| 235811 | pME_Cp_0_2_003_psaA2_prom_Cr | psaA2 promoter Chlamydomonas reinhardtii |
| 235812 | pME_Cp_0_2_004_rrn16_prom_short_Cr | rrn16 promoter short Chlamydomonas reinhardtii |
| 235813 | pME_Cp_0_2_058_Synthetic_promoter_02 | Synthetic promoter 02 based on Chlamydomonas rrn16 promoter |
| 235814 | pME_Cp_0_2_060_Synthetic_promoter_04 | Synthetic promoter 04 based on Chlamydomonas rrn16 promoter |
| 235815 | pME_Cp_0_2_061_Synthetic_promoter_05 | Synthetic promoter 05 based on Chlamydomonas rrn16 promoter |
| 235816 | pME_Cp_0_2_062_Synthetic_promoter_06 | Synthetic promoter 06 based on Chlamydomonas rrn16 promoter |
| 235818 | pME_Cp_0_2_065_Synthetic_promoter_09 | Synthetic promoter 09 based on Chlamydomonas rrn16 promoter |
| 235819 | pME_Cp_0_2_068_Synthetic_promoter_12 | Synthetic promoter 12 based on Chlamydomonas rrn16 promoter |
| 235820 | pME_Cp_0_2_070_Synthetic_promoter_14 | Synthetic promoter 14 based on Chlamydomonas rrn16 promoter |
| 235821 | pME_Cp_0_2_071_Synthetic_promoter_15 | Synthetic promoter 15 based on Chlamydomonas rrn16 promoter |
| 235822 | pME_Cp_0_2_072_Synthetic_promoter_16 | Synthetic promoter 16 based on Chlamydomonas rrn16 promoter |
| 235823 | pME_Cp_0_2_073_Synthetic_promoter_17 | Synthetic promoter 17 based on Chlamydomonas rrn16 promoter |
| 235824 | pME_Cp_0_2_075_Synthetic_promoter_19 | Synthetic promoter 19 based on Chlamydomonas rrn16 promoter |
| 235825 | pME_Cp_0_2_076_Synthetic_promoter_20 | Synthetic promoter 20 based on Chlamydomonas rrn16 promoter |
| 235826 | pME_Cp_0_2_078_Synthetic_promoter_22 | Synthetic promoter 22 based on Chlamydomonas rrn16 promoter |
| 235827 | pME_Cp_0_2_079_rbcL_promoter_Cr | rbcL promoter Chlamydomonas reinhradtii |
| 235828 | pME_Cp_0_2_080_tRNA_Glu2_promoter_Cr | tRNA Glutamate2 promoter Chlamydomonas reinhardtii |
| 235829 | pME_Cp_0_2_081_WendyII_promoter_Cr | WendyII promoter Chlamydomonas reinhardtii |
| 235831 | pME_B_0_3_009_5'UTR_dummy | 5'UTR dummy sequence used as a baseline in 5'UTR characterization |
| 235832 | pME_Cp_0_3_002_atpA_5'UTR_Cr | atpA 5'UTR Chlamydomonas reinhardtii |
| 235833 | pME_Cp_0_3_005_petB_5'UTR_Cr | petB 5'UTR Chlamydomonas reinhardtii |
| 235834 | pME_Cp_0_3_006_psaA_5'UTR_Cr | psaA 5'UTR Chlamydomonas reinhardtii |
| 235835 | pME_Cp_0_3_007_psbA_5'UTR_Cr | psbA 5'UTR Chlamydomonas reinhardtii |
| 235836 | pME_Cp_0_3_008_psbE_5'UTR_Cr | psbE 5'UTR Chlamydomonas reinhardtii |
| 235838 | pME_Cp_0_3_010_psbK_5'UTR_Cr | psbK 5'UTR Chlamydomonas reinhardtii |
| 235839 | pME_Cp_0_3_011_petA_5'UTR_Cr | petA 5'UTR Chlamydomonas reinhardtii |
| 235843 | pME_Cp_0_3_018_psaC_5'UTR_Cr | psaC 5'UTR Chlamydomonas reinhardtii |
| 235844 | pME_Cp_0_3_019_rbcL_5'UTR_Cr | rbcL 5'UTR Chlamydomonas reinhardtii |
| 235845 | pME_Cp_0_3_020_rpoC1_5'UTR_Cr | rpoC1 5'UTR Chlamydomonas reinhardtii |
| 235846 | pME_Cp_0_3_023_tufA_5'UTR_Cr | tufA 5'UTR Chlamydomonas reinhardtii |
| 235847 | pME_Cp_0_3_024_clpP_5'UTR_Cr | clpP 5'UTR Chlamydomonas reinhardtii |
| 235848 | pME_Cp_0_3_026_rpl16_5'UTR_Cr | rpl16 5'UTR Chlamydomonas reinhardtii |
| 235849 | pME_Cp_0_3_030_atpH_5'UTR_Cr | atpH 5'UTR Chlamydomonas reinhardtii |
| 235852 | pME_Cp_0_3_061_gene10_5'UTR_T7 | gene10 5'UTR T7 |
| 235853 | pME_G_0_3a_001_Placeholder | Encodes a sfGFP cassette in the 3a position |
| 235854 | pME_Cp_0_3a_001_gene10_5'UTR_T7 | gene10 5'UTR T7 3a |
| 235855 | pME_Cp_0_3a_002_petA_5'UTR_Cr | petA 5'UTR Chlamydomonas reinhardtii |
| 235856 | pME_Cp_0_3a_003_psaA_5'UTR_Cr | psaA 5'UTR Chlamydomonas reinhardtii |
| 235857 | pME_Cp_0_3a_004_psbE_5'UTR_Cr | psbE 5'UTR Chlamydomonas reinhardtii |
| 235858 | pME_Cp_0_3a_005_rbcL_5'UTR_Cr | rbcL 5'UTR Chlamydomonas reinhardtii |
| 235859 | pME_Cp_0_3IEE_001_atpE-ycf12_IEE_Cr | atpE-ycf12 IEE Chlamydomonas reinhardtii |
| 235860 | pME_Cp_0_3IEE_003_chlL-rpl36_IEE_Cr | chlL-rpl36 IEE Chlamydomonas reinhardtii |
| 235862 | pME_Cp_0_3IEE_007_psbB-psbT_IEE_Cr | psbB-psbT IEE Chlamydomonas reinhardtii |
| 235863 | pME_Cp_0_3IEE_008_psbE-rps9_IEE_Cr | psbE-rps9 IEE Chlamydomonas reinhardtii |
| 235864 | pME_Cp_0_3IEE_013_rpl2-rps19_IEE_Cr | rpl2-rps19 IEE Chlamydomonas reinhardtii |
| 235865 | pME_Cp_0_3IEE_015_rpl23-rpl2_IEE_Cr | rpl23-rpl2 IEE Chlamydomonas reinhardtii |
| 235866 | pME_Cp_0_3IEE_018_rps14-rps7_IEE_Cr | rps14-rps7 IEE Chlamydomonas reinhardtii |
| 235867 | pME_Cp_0_3IEE_021_ycf3-rps18_IEE_Cr | ycf3-rps18 IEE Chlamydomonas reinhardtii |
| 235868 | pME_Cp_0_3IEE_022_ycf4-ycf3_IEE_Cr | ycf4-ycf3 IEE Chlamydomonas reinhardtii |
| 235871 | pME_Cp_0_2-3_001_psbD_Promoter+5'UTR_Cr | psbD promoter + 5'UTR Chlamydomonas reinhardtii |
| 235872 | pME_Cp_0_2-3_002_rbcL_Promoter+5'UTR_Cr | rbcL promoter + 5'UTR Chlamydomonas reinhardtii |
| 235873 | pME_Cp_0_2-3_003_atpA_Promoter+5'UTR_Cr | atpA promoter + 5'UTR Chlamydomonas reinhardtii |
| 235874 | pME_Cp_0_2-3a_002_psbD_Promoter+5'UTR_Cr | psbD promoter + 5'UTR Chlamydomonas reinhardtii 2-3a |
| 235875 | pME_Cp_0_2-3a_003_rbcL_Promoter+5'UTR_Cr | rbcL promoter + 5'UTR Chlamydomonas reinhardtii 2-3a |
| 235877 | pME_G_0_3b_002_Placeholder | Encodes a sfGFP cassette in the N-tag position used for placeholder cloning |
| 235878 | pME_Cp_0_3b_025_Hibit-tag | HiBiT N-tag |
| 235879 | pME_Cp_0_3b_026_Flag-tag | FLAG N-tag |
| 235880 | pME_Cp_0_3b_027_HA-tag | HA N-tag |
| 235881 | pME_Cp_0_3b_028_MYC-tag | MYC N-tag |
| 235882 | pME_Cp_0_3b_029_mScarlet-I | mScarlet-I N-tag |
| 235883 | pME_Cp_0_3b_030_mCerulean | mCerulean N-tag |
| 235884 | pME_Cp_0_3b_031_mVenus | mVenus N-tag |
| 235885 | pME_Cp_0_5a_003_Hibit-tag | HiBiT C-tag |
| 235886 | pME_Cp_0_5a_004_Flag-tag | FLAG C-tag |
| 235887 | pME_Cp_0_5a_005_HA-tag | HA C-tag |
| 235890 | pME_Cp_0_5a_008_mCerulean | mCerulean C-tag |
| 235891 | pME_Cp_0_5a_009_mVenus | mVenus C-tag |
| 235892 | pME_G_0_4_001_CDS_Placeholder | Encodes a sfGFP cassette in the CDS position used for placeholder cloning |
| 235893 | pME_Cp_0_4_001_Nanoluc | Nanoluc luciferase reporter gene, codon optimized for Chlamydomonas reinhardtii |
| 235894 | pME_Cp_0_4_007_mScarlet-I | mScarlet-I reporter gene, codon optimized for Chlamydomonas reinhardtii |
| 235895 | pME_Cp_0_4_008_tdTomato | tdTomato reporter gene, codon optimized for Chlamydomonas reinhardtii |
| 235898 | pME_Cp_0_4_022_aac6-aph2-marker | aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii |
| 235900 | pME_Cp_0_4_024_CrCD-marker | Cytosine deaminase selection gene, codon optimized for Chlamydomonas reinhardtii |
| 235901 | pME_Cp_0_4_026_Arg9-marker | N-acetyl ornithine aminotransferase selection gene, codon optimized for Chlamydomonas reinhardtii |
| 235902 | pME_Cp_0_4_033_Firefly_luciferase | Firefly luciferase (red-shifted), codon optimized for Chlamydomonas reinhardtii |
| 235903 | pME_Cp_0_4_034_aadA | Aminoglycoside-3''-9-adenylyltransferase selection gene, codon optimized for Chlamydomonas reinhardtii |
| 235904 | pME_Cp_0_4_035_aphA6-Kan | Aminoglycoside-3'-phosphotransferase selection gene, codon optimized for Chlamydomonas reinhardtii |
| 235905 | pME_G_0_5_001_3'UTR_Placeholder | Encodes a sfGFP cassette in the 3'UTR position used for placeholder cloning |
| 235906 | pME_B_0_5_012_3'UTR_dummy | 3'UTR dummy sequence used as a baseline in 3'UTR characterization |
| 235907 | pME_Cp_0_5_001_psbA_3'UTR_Cr | psbA 3'UTR Chlamydomonas reinhardtii |
| 235908 | pME_Cp_0_5_005_rrnB_3'UTR_Cr | rrnB 3'UTR Chlamydomonas reinhardtii |
| 235909 | pME_Cp_0_5_006_psbA_3'UTR_Nt | psbA 3'UTR Nicotiana tabacum |
| 235910 | pME_Cp_0_5_007_atpA_3'UTR_Cr | atpA 3'UTR Chlamydomonas reinhardtii |
| 235911 | pME_Cp_0_5_009_rbcL_3'UTR+rbcL_CDS | rbcL 3'UTR + rbcL CDS Chlamydomonas reinhardtii |
| 235912 | pME_Cp_0_5_010_atpB_3'UTR_Cr | atpB 3'UTR Chlamydomonas reinhardtii |
| 235913 | pME_Cp_0_5_013_ftsH_3'UTR_Cr | ftsH 3'UTR Chlamydomonas reinhardtii |
| 235915 | pME_Cp_0_5_017_petD_3'UTR_Cr | petD 3'UTR Chlamydomonas reinhardtii |
| 235916 | pME_Cp_0_5_018_petL_3'UTR_Cr | petL 3'UTR Chlamydomonas reinhardtii |
| 235918 | pME_Cp_0_5_021_psbA_3'UTR_Cr | psbA 3'UTR Chlamydomonas reinhardtii |
| 235919 | pME_Cp_0_5_022_psbC_3'UTR_Cr | psbC 3'UTR Chlamydomonas reinhardtii |
| 235920 | pME_Cp_0_5_026_rbcL_3'UTR_Cr | rbcL 3'UTR Chlamydomonas reinhardtii |
| 235922 | pME_Cp_0_5_032_rps2_3'UTR_Cr | rps2 3'UTR Chlamydomonas reinhardtii |
| 235923 | pME_Cp_0_5_035_rps8_3'UTR_Cr | rps8 3'UTR Chlamydomonas reinhardtii |
| 235924 | pME_Cp_0_5_036_rps11_3'UTR_Cr | rps11 3'UTR Chlamydomonas reinhardtii |
| 235925 | pME_Cp_0_5_038_rps19_3'UTR_Cr | rps19 3'UTR Chlamydomonas reinhardtii |
| 235926 | pME_Cp_0_5_039_tufA_3'UTR_Cr | tufA 3'UTR Chlamydomonas reinhardtii |
| 235927 | pME_Cp_0_5_041_ycf12_3'UTR_Cr | ycf12 3'UTR Chlamydomonas reinhardtii |
| 235928 | pME_Cp_0_5_042_BMV_3'UTR_Bv | BMV 3'UTR Brome Mosaic Virus |
| 235929 | pME_Cp_0_5_043_rbcL_3'UTR_Nt | rbcL 3'UTR Nicotiana tabacum |
| 235930 | pME_Cp_0_5_045_rpoA_3'UTR_Cr | rpoA 3'UTR Chlamydomonas reinhardtii |
| 235934 | pME_Cp_0_5b_001_psbA_3'UTR_Cr | psbA 3'UTR Chlamydomonas reinhardtii |
| 235935 | pME_Cp_0_5b_002_atpB_3'UTR_Cr | atpB 3'UTR Chlamydomonas reinhardtii |
| 235936 | pME_Cp_0_5b_003_petL_3'UTR_Cr | petL 3'UTR Chlamydomonas reinhardtii |
| 235937 | pME_Cp_0_5b_005_TMV_3'UTR_Tv | TMV 3'UTR Tobacco Mosaic Virus |
| 235938 | pME_Cp_0_5b_006_ftsH_3'UTR_Cr | ftsH 3'UTR Chlamydomonas reinhardtii |
| 235940 | pME_Cp_0_6_006_psbH_5'Hom_rev_Cr | 5' Homology psbH reversee in the M2 position used for genome integration at the psbH locus |
| 235941 | pME_Cp_0_6_007_psbA_3'Hom_Cr | 3' Homology psbA in the M2 position used for genome integration at the psbA locus |
| 235942 | pME_Cp_0_6_009_rbcL_3'Hom_Cr | 3' Homology rbcL in the M2 position used for genome integration at the rbcL locus |
| 235943 | pME_Cp_0_6_013_petB_3'Hom_Cr | 3' Homology petB in the M2 position used for genome integration at the petB locus |
| 235944 | pME_Cp_0_6_019_psbD_3'Hom_Cr | 3' Homology psbD in the M2 position used for genome integration at the psbD locus |
| 235946 | pME_Cp_0_6_021_petB_5'Hom_rev_Cr | 5'Homology petB reverse in the M2 position used for genome integration at the petB locus |
| 235947 | pME_Cp_0_6_022_rbcL_5'Hom_rev_Cr | 5'Homology rbcL reverse in the M2 position used for genome integration at the rbcL locus |
| 235948 | pME_Cp_0_6_023_psbD_5'Hom_rev_Cr | 5'Homology psbD reverse in the M2 position used for genome integration at the psbD locus |
| 235949 | pME_G_0_6_021_3C1SF | 3' Connector 1 Short Forward in the M2 position used to dictate position for lvl2 assembly |
| 235950 | pME_G_0_6_022_3C2SF | 3' Connector 2 Short Forward in the M2 position used to dictate position for lvl2 assembly |
| 235951 | pME_G_0_6_023_3C3SF | 3' Connector 3 Short Forward in the M2 position used to dictate position for lvl2 assembly |
| 235952 | pME_G_0_6_024_3C4SF | 3' Connector 4 Short Forward in the M2 position used to dictate position for lvl2 assembly |
| 235953 | pME_G_0_6_025_3C5SF | 3' Connector 5 Short Forward in the M2 position used to dictate position for lvl2 assembly |
| 235955 | pME_G_0_6_027_3C7SF | 3' Connector 7 Short Forward in the M2 position used to dictate position for lvl2 assembly |
| 235956 | pME_G_0_6_004_3C1LR | 3' Connector 1 Long Reverse in the M2 position used to dictate position for lvl2 assembly |
| 235957 | pME_G_0_6_006_3C1SR | 3' Connector 1 Short Reverse in the M2 position used to dictate position for lvl2 assembly |
| 235958 | pME_G_0_6_009_3C2SR | 3' Connector 2 Short Reverse in the M2 position used to dictate position for lvl2 assembly |
| 235959 | pME_G_0_6_012_3C3SR | 3' Connector 3 Short Reverse in the M2 position used to dictate position for lvl2 assembly |
| 235960 | pME_G_0_6_015_3C5SR | 3' Connector 5 Short Reverse in the M2 position used to dictate position for lvl2 assembly |
| 235961 | pME_G_0_6_019_3C7SR | 3' Connector 7 Short Reverse in the M2 position used to dictate position for lvl2 assembly |
| 235962 | pME_G_0_5-6_006_OC3'1 | 3' Operon Connector 1 in the M2 position used to construct operons |
| 235963 | pME_G_0_5-6_007_OC3'2 | 3' Operon Connector 2 in the M2 position used to construct operons |
| 235964 | pME_G_0_5-6_008_OC3'3 | 3' Operon Connector 3 in the M2 position used to construct operons |
| 235965 | pME_G_0_5-6_009_OC3'4 | 3' Operon Connector 4 in the M2 position used to construct operons |
| 235966 | pME_G_0_5-6_010_OC3'5 | 3' Operon Connector 5 in the M2 position used to construct operons |
| 235967 | pME_G_0_5-6_011_OC3'6 | 3' Operon Connector 6 in the M2 position used to construct operons |
| 235968 | pME_G_0_5-6_012_OC3'7 | 3' Operon Connector 7 in the M2 position used to construct operons |
| 235970 | pME_Cp_0_7-8_004_Amp/ColEI_sfGFP | Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly |
| 235973 | pME_Cp_UAV_sfGFP | Universal acceptor vector with sfGFP placeholder for lvl0 assembly |
| 235975 | pME_G_E_007_UAV_sfGFP_PaqCI | Universal acceptor vector with sfGFP placeholder PaqCI compatible for lvl0 assembly |