Thomas Gonatopoulos-Pournatzis Lab: Single-Cell Exon Deletion Profiling Reveals Splicing Events That Shape Gene Expression and Cell State Dynamics
Gonatopoulos-Pournatzis T
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237451 | pLCHKOv4 | Lentiviral backbone for expressing U6-driven hybrid guide (hg)RNAs for scCHyMErA-Seq experiments. |
| 237452 | pLCHKOv5 | Lentiviral backbone for expressing doxycycline (Dox)-inducible, Pol II-driven hybrid guide (hg)RNAs. |
| 237453 | pcDNA5-miniTurbo-NRF1-FL | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full-length NRF1 isoform |
| 237454 | pcDNA5-miniTurbo-NRF1-∆ex7 | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to NRF1 isoform lacking the alternative exon-7 (∆ex7) |
| 237455 | pcDNA5-3xFLAG-NRF1-FL_WobbleMut | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full-length NRF1 isoform mutated to be resistant to NRF1 siRNA |
| 237456 | pcDNA5-3xFLAG-NRF1-∆ex7_WobbleMut | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged NRF1 isoform lacking the alternative exon-7 (∆ex7) mutated to be resistant to NRF1 siRNA |
| 237457 | pCS2+ Flag | Vector for constitutive mammalian expression of gene of interest |
| 237458 | pCS2+ Flag-SRSF3_WobbleMut | Vector for constitutive mammalian expression of Flag-tagged full-length SRSF3 mutated to be resistant to SRSF3 siRNA |
| 237459 | pET01-NRF1-Ex7-minigene | Vector for constitutive expression of NRF1 alternative exon-7 minigene |
| 237460 | pX461-ABE8e-nSpCas9 | Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA. |
| 237461 | pX461-evoCDA1-nSpCas9 | Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA. |
| 237462 | pX462-ABE8e-nSaCas9 | Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA. |
| 237463 | pX461-ABE8e-nSpCas9-Intergenic sgRNA | All-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic site |
| 237464 | pX461-evoCDA1-nSpCas9-Intergenic sgRNA | All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site |
| 237465 | pX462-ABE8e-nSaCas9-Intergenic sgRNA | All-in-one base editor expressing adenine base editor (ABE8e-nSaCas9) and sgRNA control targeting Intergenic site |
| 237466 | pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA | All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site |
| 237467 | pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA | All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site |
| 237468 | pX462-ABE8e-nSaCas9-NRF1-ss-sgRNA | All-in-one adenine base editor (ABE8e-nSaCas9) with gRNA targeting NRF1-Ex7 splice site |
| 237473 | scCHyMErA-Seq Exon Deletion Library 1 | Enables high-throughput identification of frame-preserving exons that influence transcriptional phenotypes, building on the CHyMErA system (Nature Biotechnology, 2020; PMID: 32249828). |
| 237553 | Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10 | TOPO vector for the cloning of the SpCas9 tracrRNA-CS1 - AsCas12a Direct Repeat (DR) v10 fragment into the pLCHKO_v4 hgRNA vector for scCHyMErA-Seq experiments. |