Rapid and robust generation of cardiomyocyte-specific crispants in zebrafish using the cardiodeleter system.

Keeley S, Fernandez-Lajarin M, Bergemann D, John N, Parrott L, Andrea BE, Gonzalez-Rosa JM
Cell Rep Methods. 2025 Mar 24;5(3):101003. doi: 10.1016/j.crmeth.2025.101003. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
238372	pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate	Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.
238373	pTol1-Spe-KpnI-ubb-nmKate	Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.