Rapid and robust generation of cardiomyocyte-specific crispants in zebrafish using the cardiodeleter system.
Keeley S, Fernandez-Lajarin M, Bergemann D, John N, Parrott L, Andrea BE, Gonzalez-Rosa JM
Cell Rep Methods. 2025 Mar 24;5(3):101003. doi: 10.1016/j.crmeth.2025.101003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 238308 | pTol2-cmlc2-nGFP-nCas9 | Expression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter. |
| 238309 | pTol1-U6abc_tnnt2a_cmlc2-nmKate | Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes. |
| 238372 | pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate | Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes. |
| 238373 | pTol1-Spe-KpnI-ubb-nmKate | Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate. |
| 238374 | pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate | Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes. |
| 238375 | pTol1-U6abc_amhc(myh6)_cmlc2-nmKate | Drives expression of 3 different gRNAs targeting amhc (myh6), and expression of nclear mKate in cardiomyocytes. |
| 238409 | pTol1-U6abc_erbb2_cmlc2-nmKate | Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes |
| 238410 | pTol1-U6abc_ect2_cmlc2-nmKate | Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes |