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Quantitative comparison of fluorescent proteins using protein nanocages in live cells.

Viola G, Ibrahim YW, Jacobs KA, Lemiere J, Kutys ML, Wittmann T
J Cell Sci. 2025 May 15;138(10):jcs263858. doi: 10.1242/jcs.263858. Epub 2025 May 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
239335pI3_EGFPExpresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.
239336pmStayGold_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239337pmBaoJin_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mBaoJin in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239338pmStayGold(E138D)_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239339pmStayGold_mStayGold_I3Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.
239340pmCherry_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mCherry in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239341pmScarlet-I_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet-I in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239342pmScarlet3_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239343pmScarlet3-H_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
239344pmRuby3_I3Expresses the I3-01 nanocage subunit N-terminally tagged with mRuby3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Antibodies from Article