Quantitative comparison of fluorescent proteins using protein nanocages in live cells.
Viola G, Ibrahim YW, Jacobs KA, Lemiere J, Kutys ML, Wittmann T
J Cell Sci. 2025 May 15;138(10):jcs263858. doi: 10.1242/jcs.263858. Epub 2025 May 21.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239335 | pI3_EGFP | Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs. |
| 239336 | pmStayGold_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239337 | pmBaoJin_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mBaoJin in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239338 | pmStayGold(E138D)_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239339 | pmStayGold_mStayGold_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs. |
| 239340 | pmCherry_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mCherry in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239341 | pmScarlet-I_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet-I in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239342 | pmScarlet3_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239343 | pmScarlet3-H_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs. |
| 239344 | pmRuby3_I3 | Expresses the I3-01 nanocage subunit N-terminally tagged with mRuby3 in mammalian cells. Assembles into nanocages tagged with 60 FPs. |