An auxin-inducible degron system for trypanosomes
Alexandra Klein, Alice McDowell, Christian R S Reis, Rodrigo Pontes de Lima, Danielle M N Moura, Janaina de Freitas Nascimento, Osvaldo P de Melo Neto, Mark Carrington
bioRxiv 2025.06.17.660065
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239357 | p4919 | Expression of Rice Cullin, Rice SKP1, Rice Rbx1, and G418 resistance by read through transcription after integration into the tubulin locus |
| 239358 | p5179 | Exrpession of Rice TIR1 F74->G, Rice ARF fragment and blaticidin resistance by read through transaction after integration int the PFR1 locus |
| 239359 | pPOTv7-puro-OsAID (p5109) | pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin |
| 239360 | pPOTv7-hyg-OsAID (p5110) | pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin |
| 239361 | pPOTv6-puro-3Ty:OsAID:3Ty (p5229) | pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin |
| 239362 | pPOTv6-hyg-3Ty:OsAID:3Ty (p5230) | pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin |
| 239363 | pPOTv7-hyg-AtAID:HA (p5339) | pPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin |