In vivo interaction screening reveals liver-derived constraints to metastasis.
Borrelli C, Roberts M, Eletto D, Hussherr MD, Fazilaty H, Valenta T, Lafzi A, Kretz JA, Guido Vinzoni E, Karakatsani A, Adivarahan S, Mannhart A, Kimura S, Meijs A, Baccouche Mhamedi F, Acar IE, Handler K, Ficht X, Platt RJ, Piscuoglio S, Moor AE
Nature. 2024 Aug;632(8024):411-418. doi: 10.1038/s41586-024-07715-3. Epub 2024 Jul 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239587 | pT4-U6-sgRNA-CMV-EGFP | Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker |
| 239588 | sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP | expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa |
| 239589 | sgPlxnb2-2 in pT4-U6-sgRNA-CMV-EGFP | expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa |
| 239590 | sgPlxnb2-3 in pT4-U6-sgRNA-CMV-EGFP | expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa |
| 239591 | AAV-U6-sgRNA-EF1a-LibVec | AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker |
| 239592 | AAV-U6-sgNT-1-EF1a-LibVec | expresses non-targeting (NT) guide#1 |
| 239593 | AAV-U6-sgNT-2-EF1a-LibVec | expresses non-targeting (NT) guide#2 |
| 239594 | AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec | expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa |
| 239595 | AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec | expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa |
| 239596 | AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec | expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa |
| 239597 | AAV-U6-sgPlxnb2-KO-1-EF1a-LibVec | expresses guide#1 to knock-out mouse Plxnb2, via CRISPR-Cas9 |
| 239598 | AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec | expresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9 |
| 239599 | AAV-U6-sgPlxnb2-KO-3-EF1a-LibVec | expresses guide#3 to knock-out mouse Plxnb2, via CRISPR-Cas9 |
| 239600 | AAV-U6-sgPlxnb2-KO-4-EF1a-LibVec | expresses guide#4 to knock-out mouse Plxnb2, via CRISPR-Cas9 |