John Dueber Lab: Dueber Lab plasmids 2025

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
239494	pJJB1434	Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome
239495	pKS1024	Express peroxisomal RsAS
239496	pKS1025	Express inactive peroxisomal RsAS as negative control
239497	pKS1135	Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2
239498	pKS1137	Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
239499	pKS1138	Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
239500	pKS1139	Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
239501	pKS1140	Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
239502	pKS1141	Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
239503	pKS1169	Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
239504	pKS1170	Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
239505	pKS1171	Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
239506	pKS1172	Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
239513	pKS1428	Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
239515	pKS1461	Express the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPR
239516	pKS1466	Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR
239517	pKS1469	Express the upper portion of the melvaonate pathway enzymes (MvaE and MvaS) in the peroxisome
239518	pKS1517	Express ClPT1(37-407) membrane enzyme cargo and Pex22 peroxisomal chauffeur
239519	pKS1518	Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur
239520	pKS1520	Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur
239521	pKS1531	Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
239522	pKS1574	Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
239523	pKS1575	Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
239524	pKS1576	Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
239525	pKS1577	Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
239526	pKS1601	Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
239530	pKS1612	Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
239531	pKS1613	Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
239533	pPSG803	CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
239537	pVL1176	Express Venus as ER marker
239538	pVL1279	Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
239539	pVL1280	Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
239540	pVL1281	Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
239541	pVL1337	Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
239544	pVL1727	Express Venus as ER marker
239550	pKS1619	Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR