Evolution-guided protein design of IscB for persistent epigenome editing in vivo.

Kannan S, Altae-Tran H, Zhu S, Xu P, Strebinger D, Oshiro R, Faure G, Moeller L, Pham J, Mears KS, Ni HM, Macrae RK, Zhang F
Nat Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
239828	pHS1369	WT OrufIscB mammalian expression
239829	pHS1376	WT OrufIscB omegaRNA mammalian expression
239830	pHS1505	OrufIscB-REC mammalian expression
239831	pHS_Nova	NovaIscB mammalian expression
239832	pHS2002	Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression
239833	pHS1623	WT OrufIscB E. coli expression for recombinant purification
239834	pHS1625	OrufIscB-REC E. coli expression for recombinant purification
239835	pHS2096	NovaIscB E. coli expression for recombinant purification
239836	pHS1993	OMEGAOff-KRK mammalian expression
239837	pHS2056	OMEGAOff + omegaRNA scaffold AAV expression
239838	pHS1742	OrufIscB-KRK mammalian expression