Advancing Recombinant Antibody Production in E. coli: Optimization of Expression and Purification via Dual GFP Promoter and Imaging Technology.
Korkiakoski A, Oksanen S, Huovinen T
Protein Expr Purif. 2025 Aug 28:106808. doi: 10.1016/j.pep.2025.106808.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
239861 | pLK06Hpp-Fab CR3009 | Plasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase. |