Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, Ting AY, Carr SA, Perrimon N
bioRxiv 2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240222 | pBS-GMR-eya(shRNA)_EcoRImut | pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A) |
| 240223 | pENTR_BIP-sfGFP-TurboID-KDEL | Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon) |
| 240224 | pENTR_HA-spGFP11 | Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon. |
| 240225 | pENTR_hhN-HA | Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon) |
| 240226 | pENTR_sfGFP-TurboID | Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon) |
| 240227 | pENTR_spatzle-HA | Gateway entry clone with spatzle tagged with HA (contains stop codon) |
| 240228 | pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA) | Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration. |
| 240229 | pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA) | Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration. |
| 240230 | pMT-sfGFP-TurboID-ER | Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection. |
| 240231 | pMT-sfGFP-TurboID | Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection. |
| 240232 | pUAS-sfGFP-TurboID-ER | Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration. |
| 240234 | pMT-Myc-BirA*G3-ER | Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection. |
| 240235 | pUAS-spatzle-HA | Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration. |
| 240236 | pUAS-hhN-HA | Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration. |
| 240237 | pUAS-CG6867-sfGFP | Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration. |
| 240238 | pENTR_CG6867_nostop | Gateway entry clone with CG6867 without stop codon |
| 240239 | pWal10-roe-sfGFP | Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration. |
| 240240 | pMT-EGFP-V5 | Expression of EGFP-V5 under control of copper-inducible promoter. |