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Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase.

Liu W, Duan W, Peng Z, Liao Y, Wang X, Liu R, Jing Q, Jiang H, Fan Y, Ge L, Huang L, Xing Y
Trends Biotechnol. 2025 Aug 30:S0167-7799(25)00314-2. doi: 10.1016/j.tibtech.2025.07.029. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
240286pvPE-V2Encodes second generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
240287pvPE-V3Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
240288pvPE-V4Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
240289pvPE-V3-PuroEncodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene in to mammalian cells for gene editing.
240290pvPE-V4-PuroEncodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.

Antibodies from Article