Biochemical Studies of a Cyanobacterial Halogenase Support the Involvement of a Dimetal Cofactor.
Wang ML, Glasser NR, Nair MA, Krebs C, Martin Bollinger J Jr, Balskus EP
Biochemistry. 2025 May 20;64(10):2173-2180. doi: 10.1021/acs.biochem.4c00720. Epub 2025 Apr 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240398 | pET15b-NHis6-NocL | expresses N-His tagged NocL (fatty acid activating ligase) from Nodularia sp. 06071 |
| 240399 | pCDFDuet-NHis6NocM-emptyMCS2 | expresses N-His tagged NocM (acyl carrier protein) from Nodularia sp. 06071 |
| 240400 | pETDuet-solo-NHis6-NocO | expresses N-His tagged NocO (diiron halogenase) from Nodularia sp. 06071 |
| 240401 | pETDuet-solo-NHis6-NocO-E94A | expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240402 | pETDuet-solo-NHis6-NocO-Q128A | expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240403 | pETDuet-solo-NHis6-NocO-E129A | expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240404 | pETDuet-solo-NHis6-NocO-H132A | expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240405 | pETDuet-solo-NHis6-NocO-E252A | expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240406 | pETDuet-solo-NHis6-NocO-H279A | expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240408 | pETDuet-solo-NHis6-NocO-E283A | expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |
| 240409 | pETDuet-solo-NHis6-NocO-H286A | expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid |