Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments.
Pasolli M, Meiring JCM, Conboy JP, Koenderink GH, Akhmanova A
J Cell Biol. 2025 Sep 1;224(9):e202504004. doi: 10.1083/jcb.202504004. Epub 2025 Jul 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240420 | iLID-GFP-GCN4-ppKin14 | -end oriented kinesin for optogenetic pulling experiments |
| 240421 | Vim-mCh-SspB | Tagged vimentin for optogenetic vimentin pulling experiments. |
| 240422 | KIF5A-mVenus-iLID | +end oriented kinesin for optogenetic pulling experiments |
| 240423 | Vim-mCh-FKBP | Tagged vimentin for rapalog induced pulling experiments. |
| 240424 | Halo-mitoTag | Visualization of mitochondria |
| 240425 | HA-KIF5A-FRB | +end oriented kinesin for rapalog induced pulling experiments. |