Development of a Live-Cell Imaging Assay to Elucidate Spatiotemporal Dynamics of Extracellular Vesicle Fusion with Target Cells.
van den Ende J, Defourny KAY, Rabouw HH, Tanenbaum ME, Wubbolts RW, Nolte-'t Hoen ENM
J Extracell Vesicles. 2026 Mar;15(3):e70228. doi: 10.1002/jev2.70228.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240626 | pHAGE2-palm-mScar3-10xSunTag | Expresses a fusion construct containing a palmitoylation motif, mScarlet3 and 10 repeats of the SunTag (GCN4) peptide, to luminally tag extracellular vesicles for use in EV-FUSIM assay. |
| 240630 | pCMV-VSVG-mScar3-10xSunTag | Expresses VSV-G, C-terminally fused to mScarlet3 and 10 repeats of the SunTag (GCN4) peptide, to luminally tag VSV-G-induced extracellular vesicles for use in EV-FUSIM assay. |