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In vivo production of an anti-HIV antibody in mice by non-viral gene knock-in in primate hematopoietic stem and progenitor cells.

Castelli JMP, Poljakov K, Jwa Y, Cunningham R, Cassidy ME, Gray MD, Sanchez Gaytan JN, Enstrom MR, Gastelum G, Wang Z, Linton JD, Rongvaux A, Taylor JJ, Adair JE
Mol Ther. 2026 Feb 2:S1525-0016(26)00081-X. doi: 10.1016/j.ymthe.2026.01.038. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
241002pCMVp_eGFPFor targeted gene knock-in at NHP IGH locus and constitutive expression of GFP
241003pVH4p_eGFPFor targeted gene knock-in at NHP IGH locus and B cell-specific expression of GFP
241004pVH4p_3BNC117_muSpliceFor targeted gene knock-in at NHP IGH locus and B cell-specific expression of full-length 3BNC117 following endogenous splicing
241005pVH4p_10-1074_muSpliceFor targeted gene knock-in at NHP IGH locus and B cell-specific expression of full-length 10-1074 following endogenous splicing
241007pTT3_10-1074_NHP_IgGExpresses full-length 10-1074
241008pTT3_3BNC117_NHP_IgGExpresses full-length 3BNC117

Antibodies from Article