Extracellular vesicle-mediated delivery of genetic material for transformation and CRISPR/Cas9-based gene editing in Pneumocystis murina.
Sayson SG, Ashbaugh A, Bauer LC, Smulian AG
mBio. 2025 Sep 23:e0182525. doi: 10.1128/mbio.01825-25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241014 | pSS1-mNeongreen | Expresses mNeongreen under control of Msg promoter |
| 241015 | pSS2.1-Cas9-HH-gRNADhps-HDV | Cas9 expression cassette encoding a guide RNA targeting the Dhps locus, flanked by a hammerhead (HH) ribozyme at the 5′ end and a hepatitis delta virus (HDV) ribozyme at the 3′ end |
| 241016 | pSS2.1-mNeongreen | Expresses mNeongreen under control of Msg promoter |