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The TRIP12 E3 ligase induces SWI/SNF component BRG1-beta-catenin interaction to promote Wnt signaling.

Kassel S, Yuan K, Bunnag N, Neitzel LR, Lu W, Schwarzkopf A, Maines B, Loberg MA, Xu G, Adams A, McCray AD, Cho A, Rockouski M, Orton G, Goldsmith L, Aronno MMA, Spencer ZT, Khan OM, Ye F, Williams C, Lebensohn AM, Rohatgi R, Wang X, Weiss VL, Hong CC, Kettenbach AN, Robbins DJ, Ahmed Y, Lee E
Nat Commun. 2025 Jun 5;16(1):5248. doi: 10.1038/s41467-025-60535-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
241814pCS2-Myc-TRIP12-CDExpresses Myc-tagged catalytic dead TRIP12 in mammalian cells.
241815pRP-BRG1-HA-SmBiTExpresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega HiBiT system
241816pRP-B-catenin-V5-LgBiTExpresses V5-tagged and LgBiT-tagged B-catenin for use with the Promega HiBiT system

Antibodies from Article