Photoswitchable intein for light control of covalent protein binding and cleavage.

Baloban M, Manoilov KY, Karasev MM, Verkhusha VV, Shcherbakova DM
Nat Commun. 2025 Sep 11;16(1):8263. doi: 10.1038/s41467-025-63595-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
242019	PS Intein	Blue-light-induced reconstitution of split mCherry.
242020	AAV-PS Intein	AAV version of PS Intein palsmid.
242021	PS Intein NLS-NES	Blue-light-induced reconstitution of mCherry and its relocalization to the nucleus.
242022	Split PS Intein	Blue-light-induced reconstitution of split mCherry. A T2A peptide is inserted at the natural split site of gp41-1.
242023	Split PS Intein TetR-vp16	Blue-light-induced reconstitution of the tTA transactivator via split PS Intein-mediated fusion of TetR and vp16.
242024	PS Intein Bid	Blue-light-induced protein cleavage: apoptosis via Bid activation.
242025	PS Intein/C1A Bid	PS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.
242026	Cp PS Intein tTA	Blue-light-controlled release of tTA transactivator from the cytoplasm to the nucleus.
242027	pCSII-cp PS Intein tTA Puro	A plasmid to generate a stably expressing preclonal mixture using puromycin selection.
242028	Split PS Intein tTA	Blue-light-controlled nuclear translocation of split tTA transactivator from the cytoplasm. Split version.
242029	Cp PS Intein gal4-vp16	Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus.
242030	Split PS Intein gal4-vp16	Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.
242031	Cp PS Intein dCas9-VPR	Blue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.
242032	phTERT-cp PS Intein tTA	Blue-light-controlled, hTERT-promoter-driven release of the tTA transactivator from cytoplasm to nucleus.
242033	pBIRC5-Split PS Intein-N tTA	N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.
242034	phTERT-Split PS Intein-N tTA	N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.
242035	pNF-KB-Split PS Intein-C tTA	C-terminal segment of a blue-light-controlled split PS Intein tTA gene expression system, co-expressing mCherry under an NF-KB-inducible promoter