Dominik Niopek Lab: Phage-Assisted Evolution of Allosteric Protein Switches

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
242140	PACE_AP_GIII_Titrate	PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution
242143	PACE_SP_SD4_AraC	Contains an AraC gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
242144	PACE_SP_SD4_AraC_S170_LOV	Contains an AraC-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
242145	PACE_SP_SD4_AraC_R2_S170_LOV	Contains an AraC R2 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
242146	PACE_SP_SD4_AraC_R5_S170_LOV	Contains an AraC R5 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
242148	pDest_PANCEd_AraC_sd2_WT	Expression Plasmid containing a LacI repressed sd2 WT AraC cassette
242149	pDest_PANCEd_AraC_sd2_R2_C1	Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette
242150	pDest_PANCEd_AraC_sd2_R5_C1	Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE variant cassette
242151	pDest_PANCEd_AraC_S170_LOV_sd2_WT	Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170
242152	pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1	Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170
242153	pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1	Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170