Dominik Niopek Lab: Phage-Assisted Evolution of Allosteric Protein Switches
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242138 | pLlacO1-DP6 | AraC compatible Mutagenesis Circuit (drift plasmid) |
| 242139 | PACE_AP_Pos_pBADProm | pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution |
| 242140 | PACE_AP_GIII_Titrate | PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution |
| 242141 | PACE_AP_Neg_pBADProm | pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution |
| 242143 | PACE_SP_SD4_AraC | Contains an AraC gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly |
| 242144 | PACE_SP_SD4_AraC_S170_LOV | Contains an AraC-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly |
| 242145 | PACE_SP_SD4_AraC_R2_S170_LOV | Contains an AraC R2 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly |
| 242146 | PACE_SP_SD4_AraC_R5_S170_LOV | Contains an AraC R5 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly |
| 242147 | pJUMP27_pBAD_mRFP_LVA | pBAD-mRFP Reporter Plasmid |
| 242148 | pDest_PANCEd_AraC_sd2_WT | Expression Plasmid containing a LacI repressed sd2 WT AraC cassette |
| 242149 | pDest_PANCEd_AraC_sd2_R2_C1 | Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette |
| 242150 | pDest_PANCEd_AraC_sd2_R5_C1 | Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE variant cassette |
| 242151 | pDest_PANCEd_AraC_S170_LOV_sd2_WT | Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170 |
| 242152 | pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1 | Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170 |
| 242153 | pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1 | Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170 |
| 242156 | pDest_PANCEd_AraC_R2_LDelK | Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE/RAMPhaGE Linker Library variant cassette |