Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta

Abate BA, Hahn F, Chirivì D, Betti C, Fornara F, Molloy MC, Creasey Krainer KM
bioRxiv 2025.11.09.687425 Article

These materials are included in the ENABLE® Gene Editing in planta Toolkit.

Plasmids from Article

ID	Plasmid	Purpose
242203	pGMF1-M	OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.
242204	pGMF2-M	OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.
242205	pGMF1-D	AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.
242206	pGMF2-D	AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in dicots.
242207	pGMF3	L2 binary T-DNA backbone, suitable for plant transformation.
242208	pGMF6	35Sp::eGFP:35St cassette in L1 vector backbone. Expresses a nuclear localized eGFP protein for transient plant transformation.