Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta

Abate BA, Hahn F, Chirivì D, Betti C, Fornara F, Molloy MC, Creasey Krainer KM
bioRxiv November 9, 2025 Article

Plasmids from Article

ID	Plasmid	Purpose
242203	pGMF1-M	OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.
242204	pGMF2-M	OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.
242205	pGMF1-D	AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.
242206	pGMF2-D	AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in dicots.
242207	pGMF3	L2 binary T-DNA backbone, suitable for plant transformation.
242208	pGMF6	35Sp::eGFP:35St cassette in L1 vector backbone. Expresses a nuclear localized eGFP protein for transient plant transformation.