Conversion of natural cytokine receptors into orthogonal synthetic biosensors.
Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN
Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244169 | L4308 IL-10Rb NTEVp chain (human IgG VH SS) in PolyTX-mNeonGreen | Constitutive expression of a MESA NTEVp chain encoding (N to C): hIgGVHSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S) |
| 244170 | L4312 IL-10Rb NTEVp chain (human CD8a SS) in PolyTX-mNeonGreen | Constitutive expression of a MESA NTEVp chain encoding (N to C): hCD8aSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S) |
| 244171 | L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2 | Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a |
| 244172 | L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreen | Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S) |
| 244173 | L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen | Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT) |
| 244174 | L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2 | Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6 |
| 244175 | L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreen | Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S) |
| 244176 | L4341 TNFR1 NTEVp chain (deleted MMP site) in PolyTX-mNeonGreen | Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-NTEVp(75S) |
| 244177 | L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2 | Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6 |
| 244178 | L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2 | Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6 |
| 244179 | L4868 hVEGFA-165 in pcDNA backbone | Constitutive expression of human VEGFA-165 isoform |
| 244180 | L4869 hVEGFA-121 in pcDNA backbone | Constitutive expression of human VEGFA-121 isoform |
| 244182 | L4275 hIgKLSS_hTNF in pcDNA backbone | Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence) |
| 244183 | L4276 hproTGFb1 in pcDNA backbone | Constitutive expression of human pro-TGF-beta1 (with its signal sequence) |
| 244184 | L4278 hIgKLSS_hTGFb1 in pcDNA backbone | Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence) |
| 244185 | L4300 hIL10 in pcDNA backbone | Constitutive expression of human IL-10 (with its signal sequence) |
| 244186 | L4385 dsRedExpress2 reporter, IL-10Rb NTEVp, IL-10Ra CTEVp in PiggyBac Transposon Vector | PiggyBac transposon vector for expression of dsRedExpress2 under synTF promoter; constitutive expression of IL-10Rb NTEVp chain, IL-10Ra CTEVp chain, and mNeonGreen-P2A-PuroR selection marker |
| 244187 | L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector | PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker |
| 244188 | L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), VEGFR1 CTEVp chain with ZF6a in MGEV | Multigene vector for constitutive expression of mNeonGreen, VEGFR2 NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a |
| 244189 | L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV | Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a |
| 244190 | L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV | Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a |
| 244191 | L4872 VP64-intN in PolyTX-mNeonGreen | Constitutive expression of VP64-intN split synTF component |
| 244192 | L4873 intC-ZF9 in PolyTX-mTagBFP2 | Constitutive expression of intC-ZF9 split synTF component |
| 244193 | L3928 Filler DNA | Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden |
| 244194 | L4427 IL-10Ra CTEVp chain with cJun in TUPV3 | Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hcJun |
| 244195 | L4426 IL-10Ra CTEVp chain with BATF in TUPV3 | Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hBATF |
| 244196 | L4428 IL-10Ra CTEVp chain with Tbet in TUPV3 | Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hTbet |
| 244197 | L4437 x6TRE (cJun and AP-1) Ben DsRed-Express2 in TUPV1 | Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (AP-1 binding sites) |
| 244199 | L4459 x6BRE (BATF) Ben DsRed-Express2 in TUPV1 | Expression of a DsRed-Express2 reporter under a BATF-responsive promoter |
| 244200 | L4505 x6TRE2 (cJun2) Ben DsRed-Express2 in TUPV1 | Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (cJun homodimer binding sites) |
| 244201 | L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1 | Expression of a DsRed-Express2 reporter under an NFAT-responsive promoter |
| 244202 | L4826 ZF1+ZF6 x12c interspersed Ben DsRed-Express2 in TUPV1 | Expression of a DsRed-Express2 reporter under a hybrid synTF-responsive promoter |
| 244203 | L4832 ZF1+ZF6 x6c interspersed Ben DsRed-Express2 in TUPV1 | Expression of a DsRed-Express2 reporter under a hybrid synTF-responsive promoter |