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Conversion of natural cytokine receptors into orthogonal synthetic biosensors.

Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN
Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
244169L4308 IL-10Rb NTEVp chain (human IgG VH SS) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): hIgGVHSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)
244170L4312 IL-10Rb NTEVp chain (human CD8a SS) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): hCD8aSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)
244171L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a
244172L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S)
244173L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT)
244174L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
244175L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S)
244176L4341 TNFR1 NTEVp chain (deleted MMP site) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-NTEVp(75S)
244177L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
244178L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6
244179L4868 hVEGFA-165 in pcDNA backboneConstitutive expression of human VEGFA-165 isoform
244185L4300 hIL10 in pcDNA backboneConstitutive expression of human IL-10 (with its signal sequence)
244191L4872 VP64-intN in PolyTX-mNeonGreenConstitutive expression of VP64-intN split synTF component
244192L4873 intC-ZF9 in PolyTX-mTagBFP2Constitutive expression of intC-ZF9 split synTF component

Antibodies from Article