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Conversion of natural cytokine receptors into orthogonal synthetic biosensors.

Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN
Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
244169L4308 IL-10Rb NTEVp chain (human IgG VH SS) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): hIgGVHSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)
244170L4312 IL-10Rb NTEVp chain (human CD8a SS) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): hCD8aSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)
244171L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a
244172L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S)
244173L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT)
244174L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
244175L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S)
244176L4341 TNFR1 NTEVp chain (deleted MMP site) in PolyTX-mNeonGreenConstitutive expression of a MESA NTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-NTEVp(75S)
244177L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
244178L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6
244179L4868 hVEGFA-165 in pcDNA backboneConstitutive expression of human VEGFA-165 isoform
244180L4869 hVEGFA-121 in pcDNA backboneConstitutive expression of human VEGFA-121 isoform
244182L4275 hIgKLSS_hTNF in pcDNA backboneConstitutive expression of human mature TNF (with the human IgK light chain secretion sequence)
244183L4276 hproTGFb1 in pcDNA backboneConstitutive expression of human pro-TGF-beta1 (with its signal sequence)
244184L4278 hIgKLSS_hTGFb1 in pcDNA backboneConstitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)
244185L4300 hIL10 in pcDNA backboneConstitutive expression of human IL-10 (with its signal sequence)
244186L4385 dsRedExpress2 reporter, IL-10Rb NTEVp, IL-10Ra CTEVp in PiggyBac Transposon VectorPiggyBac transposon vector for expression of dsRedExpress2 under synTF promoter; constitutive expression of IL-10Rb NTEVp chain, IL-10Ra CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
244187L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon VectorPiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
244188L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), VEGFR1 CTEVp chain with ZF6a in MGEVMultigene vector for constitutive expression of mNeonGreen, VEGFR2 NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a
244189L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEVMultigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a
244190L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEVMultigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a
244191L4872 VP64-intN in PolyTX-mNeonGreenConstitutive expression of VP64-intN split synTF component
244192L4873 intC-ZF9 in PolyTX-mTagBFP2Constitutive expression of intC-ZF9 split synTF component
244193L3928 Filler DNAEmpty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden
244194L4427 IL-10Ra CTEVp chain with cJun in TUPV3Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hcJun
244195L4426 IL-10Ra CTEVp chain with BATF in TUPV3Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hBATF
244196L4428 IL-10Ra CTEVp chain with Tbet in TUPV3Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hTbet
244197L4437 x6TRE (cJun and AP-1) Ben DsRed-Express2 in TUPV1Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (AP-1 binding sites)
244199L4459 x6BRE (BATF) Ben DsRed-Express2 in TUPV1Expression of a DsRed-Express2 reporter under a BATF-responsive promoter
244200L4505 x6TRE2 (cJun2) Ben DsRed-Express2 in TUPV1Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (cJun homodimer binding sites)
244201L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1Expression of a DsRed-Express2 reporter under an NFAT-responsive promoter
244202L4826 ZF1+ZF6 x12c interspersed Ben DsRed-Express2 in TUPV1Expression of a DsRed-Express2 reporter under a hybrid synTF-responsive promoter
244203L4832 ZF1+ZF6 x6c interspersed Ben DsRed-Express2 in TUPV1Expression of a DsRed-Express2 reporter under a hybrid synTF-responsive promoter

Antibodies from Article