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Beth Rowan Lab: Monitoring the stability of transgene expression in lettuce using the RUBY reporter.

Unpublished

Plasmids from Article

ID Plasmid Purpose
244448pL0M-PU-pLsUBILevel 0 Golden Gate "PU" module containing the domesticated sequence of the promoter of the polyubiquitin 4 gene (LOC111919935) from lettuce (Lactuca sativa)
245484pL0M-T-tLsUBILevel 0 Golden Gate "T" module containing the sequence of the terminator of the polyubiquitin 4 gene (LOC111919935) from lettuce (Lactuca sativa)
245485pL0M-SC-RUBYGolden Gate Level 0 "SC" RUBY module
245486pR2B5pL1-R2-pAtUBI-RUBY-tRBCS (Golden Gate Level 1 module with a transcription unit expressing RUBY from the AtUBI promoter with the tRBC terminator for R2 position in Level 2 vector)
245487pR2B6pL1-R2-pLsUBI-RUBY-tLsUBI (Golden Gate Level 1 module with a transcription unit expressing RUBY from the LsUBI promoter with the tLsUBI terminator for R2 position in Level 2 vector)
245488pATUBRBinary Plasmid for plant transformation expressing RUBY from the Arabidopsis thaliana polyubiquitin 10 gene promoter and the terminator from the pea RBCS gene. Kanamycin plant selection
245489pLSUBRBinary Plasmid for plant transformation expressing RUBY from the Lactuca sativa polyubiquitin 4 gene promoter and the terminator from the same gene. Kanamycin plant selection
245490pJD849Binary Plasmid for plant transformation expressing RUBY from the 35S promoter and the terminator from the Arabidopsis thaliana HSP gene. Kanamycin plant selection

Antibodies from Article