Joe Zhang Lab: Protocol for high-efficiency generation of iPSCs stably expressing Cas9-EGFP using the selection by essential gene exon knockin method.

Zhang Y, Yang H, Yang Y, Lu Z, Cheng L, Tan H, Zhang JZ
STAR Protoc. 2025 Jul 4;6(3):103928. doi: 10.1016/j.xpro.2025.103928. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
249545	pCas9-EGFP_SLEEK	Insert the Cas9-EGFP into exon 9 of GAPDH for stable expression.
249575	pX458_GAPDH-sgRNA	Expresses SpCas9, GFP, and sgRNA targeting GAPDH.