Transgene-free genome editing in citrus and poplar trees using positive and negative selection markers.
Rocha DC, Omoregbee MO, Contiliani DF, Mandlik R, Li G, Mascoveto J, Coleman G, Culver JN, Leal DR, de Souza AA, Qi Y
Plant Cell Rep. 2025 Oct 22;44(11):244. doi: 10.1007/s00299-025-03627-2.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 245873 | pYPQ131-FCY-UPP | Golden gate entry vector carrying expression cassette for genes FCY and UPP |
| 245875 | pYPQ132B-CsALSgR1.1 | Golden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene |
| 245876 | pYPQ133B-CsALSgR1.2 | Golden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene |
| 245877 | pYPQ132B-TLS-CsALSgR1.1 | Golden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequence |
| 245878 | pYPQ133B-TLS-CsALSgR1.2 | Golden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequence |
| 245879 | pYPQ134B-TLS | Golden gate entry vector carrying TLS mobile RNA sequence |
| 245880 | pYPQ132B-PtALSgRNA | Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene |
| 245881 | pYPQ132B-TLS-PtALSgRNA | Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene along with TLS mobile RNA sequence |
| 245882 | pYPQ133B-TLS | Golden gate entry vector carrying TLS mobile RNA sequence |