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Incorporation of cofilin into rods depends on disulfide intermolecular bonds: implications for actin regulation and neurodegenerative disease.

Bernstein BW, Shaw AE, Minamide LS, Pak CW, Bamburg JR
J Neurosci. 2012 May 9;32(19):6670-81. doi: 10.1523/JNEUROSCI.6020-11.2012. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
245966huCof C39A-mRFPExpression of cof with one of 4 cys mutated to ala. Incorporates into cofilactin rods
245967huCof C39S-mRFPExpression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods
245968huCof C139A-mRFPExpression of cof with one of 4 cys mutated to ala. Incorporates into cofilactin rods
245969huCof C139S-mRFPExpression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods
245970huCof C147A-mRFPExpression of cof with one of 4 cys mutated to ala .Incorporates into cofilactin rods
245971huCof C147S-mRFPExpression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods
245972huCof C39A,C147A-mRFPExpression of cof with two of 4 cys mutated to A. Does NOT incorporate into cofilin-actin rods .
245974huCof K95Q-mRFPExpression of cofilin with mutation in one actin binding domain

Antibodies from Article