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Engineered ADARs enable single-nucleotide resolution DNA A-to-G editing without bystander effects

Im HW, Jeong B, Lee Y, Oh YE, CJung C, Kim YW, Uhm H, Bae S
bioRxiv 2025.07.19.664718 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246011snuABE3.1snuABE3.1 composed of PhADAR(E438Q) fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
246013snuABE4.1snuABE4.1 composed of PhADAR(E438Q, I536S, E637F) with 10a.a truncation fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
246014snuABE4.2snuABE4.1 with MLH1-SB fused to the C-terminus via P2A.

Antibodies from Article