Conversion of IscB and Cas9 into RNA-guided RNA editors.
Xu C, Niu X, Sun H, Yan H, Tang W, Ke A
Cell. 2025 Aug 18:S0092-8674(25)00854-2. doi: 10.1016/j.cell.2025.07.032.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246430 | pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA | All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included. |
| 246435 | pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron | Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS. |
| 246436 | pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA | Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments. |
| 246438 | NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide | All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene. |