Structural basis for L-isoaspartyl-containing protein recognition by the PCMTD1 cullin-RING E3 ubiquitin ligase.
Pang EZ, Zhao B, Flowers C, Oroudjeva E, Winter JB, Pandey V, Sawaya MR, Wohlschlegel J, Loo JA, Rodriguez JA, Clarke SG
bioRxiv [Preprint]. 2025 May 21:2025.05.21.654933. doi: 10.1101/2025.05.21.654933.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246496 | pET-28a(+) PCMT1 H58 A214 | Recombinant protein expression of PCMT1 H58 A214 |
| 246504 | pET24+ CUL5(1-384) | Recombinant protein expression of CUL5(1-384) |
| 246505 | pMAPLe4 PCMTD1 F57 F217 F220 | Recombinant protein expression of PCMTD1 F57 F217 F220 |
| 246506 | pMAPLe4 PCMTD1 T62 H67 M68 | Recombinant protein expression of PCMTD1 T62 H67 M68 |
| 246507 | pMAPLe4 PCMTD1 PCMTD1(∆ESB-ASB9) | Recombinant protein expression of PCMTD1(∆ESB-ASB9) |