Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing.
Llorens-Gamez JJ, Garcia-Cano PJ, Rico-Rodrigo S, Duyos-Casanova L, Toledano-Franco S, Carbonell A
Plant J. 2026 Jan;125(1):e70665. doi: 10.1111/tpj.70665.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246715 | pENTR-BS-AtMIR390a-A18G-B/c | Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs. |
| 246716 | pMDC32B-BS-AtMIR390a-A18G-B/c | Cloning amiRNA sequences in AtMIR390a-A18G precursors for in planta expression |