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A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging.

Ding Y, He W, Wang K, Xue F, Zheng K, Zhao S, Qi D, Li T, Xia P, Pan L, Chen L, Chen Z, Yuan L, Xu P
Nat Methods. 2025 Nov 26. doi: 10.1038/s41592-025-02962-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246871pRSETA-mScarlet3-S2Expression of mScarlet3-S2
246872H2B-mScarlet3-S2Expression of mScarlet3-S2. This contruct is used for imaging, photobleaching and photochromic behavior measurements.
246873Lifeact-mScarlet3-S2Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging.
246874mScarlet3-S2-Sec61βExpression of mScarelt3-S2 fused with Sec61β. This construct is used for imaging.
246875mScarlet3-S2-Lamin A/CExpression of mScarlet3-S2 fused with Lamin A/C. This construct is used for imaging.

Antibodies from Article