Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design
Drepanos LM, Srikanth S, Kaplan EG, Shah ST, Escude Velasco B, Merzouk S, Doench JG
bioRxiv 2025.08.26.672375; doi: https://doi.org/10.1101/2025.08.26.672375
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247026 | Jacquere (split-vector) | Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs. |
| 247027 | Jacquere (all-in-one vector) | Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs. |
| 247028 | Julianna (split-vector) | Mouse genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs. |