NanoBondy Reaction through NeissLock Anhydride Allows Covalent Immune Cell Decoration.
Vankayala LR, Adoni KR, Lim SYT, Dam T, Dushek O, Thalassinos K, Howarth MR
Bioconjug Chem. 2026 Feb 18;37(2):281-292. doi: 10.1021/acs.bioconjchem.5c00519. Epub 2026 Jan 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247051 | pET28a-NanoBondy 2H5(R72C) | For expression of covalently reactive anti-CD45 2H5-derived NanoBondy (R72C clamp site, 9 residue linker) in bacterial cytoplasm |
| 247052 | pLEXm-hCD45d | For mammalian expression of human CD45 domains 1 and 2 with C-terminal Maltose-Binding Protein fusion for secretion into the medium |