Continuous targeted hypermutation with a tunable mutation window.
Lee C, Kim S
Nat Commun. 2025 Nov 25. doi: 10.1038/s41467-025-66736-2.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247069 | pCDF-cascade | L-arabinose inducible E.coli cascade operon inserted in pCDF vector |
| 247071 | pMut3_pm32B | L-arabinose inducible pmCDA-XTEN32-CasB(Cas11)-GSSG-UGI |
| 247072 | pMut12_apo64C | L-arabinose inducible rAPOBEC-XTEN64-CasC(Cas7)-GSSG-UGI |
| 247073 | pMut19_tadDE16B | L-arabinose inducible TadDE-XTEN16-CasB(Cas11)-GSSG-UGI |
| 247074 | pMut20_tadDE32B | L-arabinose inducible TadDE-XTEN32-CasB(Cas11)-GSSG-UGI |
| 247075 | pGRNA1_105nt | L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets pheS*_codon |
| 247076 | pGRNA2I_LacZB1 | L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ B1 site |
| 247079 | pGRNA2II_LacZT1 | L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ T1 site |
| 247080 | pGRNA3_LacZ_IB1_IIT1 | L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and T1 site. riboJ/RNaseIII site are encoded between two crRNA |
| 247081 | pGRNA3_LacZ_B1_IIB5 | L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and B5 site |