The Arabidopsis CYSTM alpha 5' UTR Increases Protein Production from Transgenes in Plants and Bacteria.
Khanduja JS, Wu X, Li J, Searle IR
Genes (Basel). 2026 Apr 28;17(5):520. doi: 10.3390/genes17050520.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247490 | pluc HYG 5’ CYSTM α | Plasmid pluc 5′ CYSTM α contains a 79-nt Arabidopsis 5′ UTR fragment upstream of Firefly luciferase, functioning as a potent translational enhancer in plant assays. |
| 247491 | pluc HYG 5’ TMV Ω | Plasmid pluc 5′ TMV Ω contains the 67-nt TMV Ω translational enhancer placed upstream of Firefly luciferase, optimized for boosting expression in plant transient and stable expression assays. |
| 247492 | pluc HYG 5’ CYSTM1 | Plasmid pluc 5′ CYSTM1 contains the full 110-nt Arabidopsis CYSTM1 5′ UTR upstream of Firefly luciferase, serving as a translational enhancer in plant transient assays |
| 247493 | p35S:RUBY 5’ CYSTM α | Plasmid p35S:RUBY 5’ CYSTM α contains a 79-nt 5′ CYSTM α sequence placed directly upstream of the CYP76AD1 coding region (first gene in the RUBY cassette), intended to boost translation efficiency |
| 247568 | pluc HYG | Plasmid containing a firefly luciferase reporter gene and hygromycin resistance cassette for selection in plant transformation experiments. |