Connectome-seq: high-throughput mapping of neuronal connectivity at single-synapse resolution via barcode sequencing.
Chen D, Isakova A, Wan Z, Wagner MJ, Wu Y, Zhao BS
Nat Methods. 2026 Mar 12. doi: 10.1038/s41592-026-03026-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247715 | hSyn-PreSynBar-hSyn-PreRNA short | SynBar protein and RNA construct used in HEK and neuron culture experiment. |
| 247716 | hSyn-PostSynBar-hSyn-PostRNA short | SynBar protein and RNA construct used in HEK and neuron culture experiment. |
| 247717 | hSyn-PreSynBar-hSyn-PreRNA barcode landing pad | The set of SynBar constructs used in the optimized connectome-seq experiments |
| 247718 | hSyn-Cre-hSyn-PreRNA barcode landing pad (PPIB) | The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment. |
| 247719 | hSyn-DIO-PostSynBar-P2A-mCherry | The set of SynBar constructs used in the optimized connectome-seq experiments. |
| 247720 | hSyn-Cre-hSyn-PostRNA barcode landing pad (dapB) | The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment. |
| 247721 | sCAG-DIO-oScarlet3 | Used for AAV1 tracing experiment. |