Connectome-seq: High-throughput Mapping of Neuronal Connectivity at Single-Synapse Resolution via Barcode Sequencing.
Chen D, Isakova A, Wan ZJ, Wagner MJ, Wu Y, Zhao BS
bioRxiv [Preprint]. 2025 May 26:2025.02.13.638129. doi: 10.1101/2025.02.13.638129.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247715 | hSyn-PreSynBar-hSyn-PreRNA short | SynBar protein and RNA construct used in HEK and neuron culture experiment. |
| 247716 | hSyn-PostSynBar-hSyn-PostRNA short | SynBar protein and RNA construct used in HEK and neuron culture experiment. |
| 247717 | hSyn-PreSynBar-hSyn-PreRNA barcode landing pad | The set of SynBar constructs used in the optimized connectome-seq experiments |
| 247718 | hSyn-Cre-hSyn-PreRNA barcode landing pad (PPIB) | The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment. |
| 247719 | hSyn-DIO-PostSynBar-P2A-mCherry | The set of SynBar constructs used in the optimized connectome-seq experiments. |
| 247720 | hSyn-Cre-hSyn-PostRNA barcode landing pad (dapB) | The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment. |
| 247721 | sCAG-DIO-oScarlet3 | Used for AAV1 tracing experiment. |