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Feature-specific threat coding in lateral septum guides defensive action.

Mazo DLB, Pasqualini AL, Wu SJ, Berger MZC, Reid CM, Brito SI, Qiu S, Levitt P, Anthony TE, Fishell G
Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6831193. doi: 10.21203/rs.3.rs-6831193/v1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248662pAAV_EF1a-FLEx(FRT)-stGtACR2:eGFP (soma-targeted)Expresses a soma-targeted GtACR2 in Flp-expressing cells under the EF1a promoter for neuronal inhibition.
248663pAAV_CAG(del)-FLEx(loxP)-mCherry(nuclear)-2A-N2cGHelper virus for monosynaptic tracing; Expresses nuclear mCherry and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.
248664pAAV_CAG(del)-FLEx(loxP)-eYFP(nuclear)-2A-N2cGHelper virus for monosynaptic tracing; Expresses nuclear eYFP and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.
248665pAAV_CAG(del)--FLEx(loxP)-splitTVA:HAHelper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.
248667pAAV_CAG(del)-FLEx(loxP)-ARch3.0:EYFPExpresses Archaerhodopsin3 tagged to eYFP in Cre-expressing cells under the CAG promoter for neuronal inhibition.

Antibodies from Article