Jennifer Doudna Lab: Recurrent acquisition of nuclease-protease pairs in antiviral immunity
Doudna J et al.
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248931 | pTet_PflHamMAB | Hachiman from P. fluorescens under pTet control |
| 248933 | pTet_PflHamMA(S157A)B | Hachiman from P. fluorescens under pTet control with HamA mutated (S157A) |
| 248934 | pTet_PflHamMAB(D207A) | Hachiman from P. fluorescens under pTet control with HamB mutated (D207A) |
| 248935 | pTet_PflHamM(I118K)AB | Hachiman from P. fluorescens under pTet control with HamM mutated (I118K) |
| 248937 | pTet_PflHamM(I118K,I218K)AB | Hachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K) |
| 248938 | pTet_PflHamMA(deltaA)B | Hachiman from P. fluorescens under pTet control with the dHamA domain removed |
| 248939 | pTet_PflHamMA(S157A)(deltaA)B | Hachiman from P. fluorescens under pTet control with the dHamA domain removed and with a protease S157A mutation |
| 248940 | pTet_PflHamMA(-cJun)B | Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker |
| 248941 | pTet_PflHamMA(S157A)(-cJun)B | Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker and the protease mutated (S157A) |
| 248942 | pTet_PflHamM(pre-cut)AB | Hachiman from P. fluorescens under pTet control with HamM programmed breaks in the peptide backbone at I118 and I218 |
| 248943 | exp_PflHamM | His-MBP-HamM from P. fluorescens for overexpression. |
| 248946 | pLG017_Avs1a(I234K) | AVAST from E. piriflorinigrans with Avs1a mutated (I234K) |
| 248947 | pLG017_Avs1a(I134K,I234K) | AVAST from E. piriflorinigrans with Avs1a mutated (I134K, I234K) |
| 248948 | pLG017_FLAG-Avs1a | AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a |
| 248949 | pLG017_FLAG-Avs1a(I134K,I234K) | AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K) |
| 248950 | pLG017_Avs1a_chimera | AVAST from E. piriflorinigrans with Avs1a replaced with Avs1a from Citrobacter Avs1a |
| 248952 | pJEX_lambdaterminase | phage lambda large terminase subunit under control of a crystal violet inducible promoter |
| 248953 | pTet_KaeCanABC | CanABC from K. aerogenes under control of pTet and the native promoter |
| 248954 | pTet_KaeCanA(C187A)BC | CanABC from K. aerogenes under control of pTet and the native promoter, with CanA mutated (C187A) |
| 248955 | pTet_KaeCanABC(H271A) | CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (H271A) |
| 248956 | pTet_KaeCanABC(G104K) | CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G104K) |
| 248957 | pTet_KaeCanABC(G189K) | CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G189K) |
| 248958 | pTet_KaeCanABC(G104K,G189K) | CanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K) |
| 248959 | exp_KaeCanAB | His-MBP-CanAB from K. aerogenes for overexpression. |
| 248960 | exp_KaeCanC | His-MBP-CanC from K. aerogenes for overexpression. |