Reduced Cas9 transgene silencing by incorporation of intron sequences.
Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, Prolo LM, Bassik MC
Nat Commun. 2025 Nov 27;16(1):10656. doi: 10.1038/s41467-025-65669-0.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249132 | pSA071 Cas9-BFP | Overexpression of SpCas9-BFP and blasticidin resistance gene. |
| 249133 | pSA072 Cas9-HBB_IVS2-BFP | Overexpression of SpCas9-BFP with HBB IVS2 intron after Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249134 | pSA073 HBB_IVS2-Cas9-BFP | Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249135 | pSA074 Cas9-BFP/HBB_IVS2/BFP | Overexpression of SpCas9-BFP with HBB IVS2 intron within BFP coding sequence and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249136 | pSA075 Cas9-BFP-HBB_IVS2 | Overexpression of SpCas9-BFP with HBB IVS2 intron in 3' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249137 | pSA076 Cas9/HBB_IVS2/Cas9-BFP | Overexpression of SpCas9-BFP with HBB IVS2 intron within Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249138 | pSA081 BFP | Overexpression of BFP. Contains Cp36 recombination site. |
| 249139 | pSA089 AARS1_IVS11-Cas9-BFP | Overexpression of SpCas9-BFP with AARS IVS11 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249140 | pSA090 EIF4A3_IVS2-Cas9-BFP | Overexpression of SpCas9-BFP with EIF4A3 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249141 | pSA091 PSMD14_IVS2-Cas9-BFP | Overexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249142 | pSA092 CCT3_IVS13-Cas9-BFP | Overexpression of SpCas9-BFP with CCT3 IVS13 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249143 | pSA093 PFDN2_IVS3-Cas9-BFP | Overexpression of SpCas9-BFP with PFDN2 IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249144 | pSA094 UBA1_IVS20-Cas9-BFP | Overexpression of SpCas9-BFP with UBA1 IVS20 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249145 | pSA095 EEF2_IVS9-Cas9-BFP | Overexpression of SpCas9-BFP with EEF2 IVS9 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249146 | pSA096 PSMD4_IVS6-Cas9-BFP | Overexpression of SpCas9-BFP with PSMD4 IVS6 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249147 | pSA097 HNRNPK_IVS3-Cas9-BFP | Overexpression of SpCas9-BFP with HNRNPK IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249148 | pSA098 RPL18A_IVS4-Cas9-BFP | Overexpression of SpCas9-BFP with RPL18A IVS4 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249149 | pSA099 PGK1_IVS3-Cas9-BFP | Overexpression of SpCas9-BFP with PGK1 IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249150 | pSA100 RPS12_IVS1-Cas9-BFP | Overexpression of SpCas9-BFP with RPS12 IVS1 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249151 | pSA127 A2UCOE-Cas9-BFP | Overexpression of SpCas9-BFP and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site. |
| 249152 | pSA128 A2UCOE-HBB_IVS2-Cas9-BFP | Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site. |
| 249153 | pSA143 Cas9-BFP with core cHS4 insulators | Overexpression of SpCas9-BFP and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site. |
| 249154 | pSA144 HBB_IVS2-Cas9-BFP with core cHS4 insulators | Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site. |
| 249155 | pPD231 PPH-T2A-dCas9-NFZ-P2A-BFP | Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249156 | pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP | Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site. |
| 249157 | pPD684 EnAsCas12a-BFP | Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains Cp36 recombination site. |
| 249158 | pPD685 HBB_IVS2-EnAsCas12a-BFP | Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains Cp36 recombination site. |
| 249159 | pPD686 A2UCOE-EnAsCas12a-BFP | Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site. |
| 249160 | pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP | Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site. |