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Astrocyte-microglia crosstalk through Hevin and Toll-like receptor signaling controls developmental thalamocortical synapse refinement.

Ramirez JJ, Hardin EJ, Sakers K, Kim J, Colon Ortiz C, Savage JT, Hanamsagar R, Block CL, Singh SK, Bilbo SD, Eroglu C
Neuron. 2026 Feb 19:S0896-6273(25)00984-5. doi: 10.1016/j.neuron.2025.12.028. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
249174pAP-Tag5-IgKsp-mHevin-Myc-HisOverexpression of full length mouse Sparcl1 with Myc and 6xHis tag
249175pAP-Tag5-IgKsp-mSLF-Myc-HisOverexpression of C-terminal fragment of mouse Sparcl1 with Myc and 6xHis tag
249176pAP-Tag5-IgKsp-DE-Myc-HisOverexpression of mouse Sparcl1 fragment a.a. 330-550 with Myc and 6xHis tag
249177pAP-Tag5-IgKsp-mHevin∆DE-Myc-HisOverexpression of mouse Sparcl1 with deletion of a.a. 330-550 with Myc and 6xHis tag
249178pAAV-GfaABC1D-mHevin-Myc-HisAAV mediated overexpression of full length mouse Sparcl1
249179pAAV-GfaABC1D-mSLF-Myc-HisAAV mediated overexpression of C-terminal fragment of mouse Sparcl1
249180pAAV-GfaABC1D-mDE-Myc-HisAAV mediated overexpression of mouse Sparcl1 a.a. 330-550 fragment
250167pAAV-hsyn1-synaptophysin-mCherry-V5Neuronal overexpression of synaptophysin fused to mCherry
250168pAAV-gfaABC1D-mCherry-CAAXAAV mediated overexpression of membrane targeted mCherry

Antibodies from Article