Formation of a complex between TMEM217 and the sodium-proton exchanger SLC9C1 is crucial for mouse sperm motility and male fertility.
Iida-Norita R, Miyata H, Ninomiya A, Emori C, Kamoshita M, Pan C, Wang H, Ikawa M
Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2513924122. doi: 10.1073/pnas.2513924122. Epub 2025 Oct 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249335 | pClgn1.1-mouse Tmem217-3xFlag | Expression vector of mouse Tmem217 tagged with 3xFLAG at C-terminus under the mouse Clgn promoter. |
| 249409 | pCAG-mouse Tmem217-3xFlag | Expression vector of mouse Tmem217 tagged with 3xFLAG at C-terminus under the CAG promoter. |
| 249410 | pCAG-human TMEM217-PA | Expression vector of human TMEM217 isoform 3 tagged with PA at C-terminus under the CAG promoter. |
| 249411 | pCAG-human SLC9C1-3xFlag | Expression vector of human SLC9C1 tagged with 3xFLAG at C-terminus under the CAG promoter. |
| 249412 | pCAG-mouse Slc9c1-PA | Expression vector of mouse SLC9C1 tagged with PA at C-terminus under the CAG promoter |
| 249413 | pCAG-mouseSlc9c1(ΔExchanger/ΔVSD (S1-S3))-PA | Expression vector of mouse Slc9c1(aa733-1175) tagged with PA at C-terminus under the CAG promoter |
| 249415 | pCAG-mouse Slc9c1 (C-term)-PA | Expression vector of mouse SLC9C1 (aa1026-1175) tagged with PA at C-terminus under the CAG promoter. |
| 249417 | pX459-TMEM217-gRNA#1 | pX459-gRNA#1 targeting the 5′ coding region of human TMEM217 |
| 249418 | pX459-TMEM217-gRNA#2 | pX459 plasmid containing gRNA#2 targeting the end of the coding exon of human TMEM217 |