CRISPR targeting of H3K4me3 activates gene expression and unlocks centromere-proximal crossover recombination in Arabidopsis.

Binenbaum J, Adamkova V, Fryer H, Xu L, Gorringe N, Wlodzimierz P, Burns R, Papikian A, Jacobsen SE, Henderson IR, Harris CJ
Nat Commun. 2025 Oct 31;16(1):9587. doi: 10.1038/s41467-025-65167-3. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
249560	Suntag_PRDM9_FWA_g4	dCas9_Suntag_PRDM9 targeting FWA promoter
249561	Suntag_PRDM9_No_g	Suntag_PRDM9 no guide
249562	Suntag_dPRDM9_FWA_g4	dCas9_dPRDM9 catalytically dead version
249570	Suntag_PRDM9_LRCen3g	dCas9_PRDM9 targeting the centromere 3