Simon Newstead Lab: Newstead Lab Plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250412 | pSBinit-Syb-BC10(Y107A/W108S) | Expression of Anti-SLC66A2 Sybody Non-Binding Variant in Bacteria |
| 250413 | pSBinit-Syb-BC10(Y35A/Y107A/W108S) | Expression of Anti-SLC66A2 Sybody Non-Binding Variant in Bacteria |
| 250414 | pSBinit-Syb-BC10(R60A/Y107A/W108S) | Expression of Anti-SLC66A2 Sybody Non-Binding Variant in Bacteria |
| 250415 | pSBinit-Syb-BC10(Y35A/Y56A/R60A/I61T/H63A/T105A/Y107A/W108S) | Expression of Anti-SLC66A2 Sybody Non-Binding Variant in Bacteria |
| 250416 | pLexM-HsPQLC1(16-271)-FLAG | Expression of C-terminally FLAG-Tagged, N-Terminally Truncated (?1-15) HsPQLC1 in Mammalian Cells |
| 250417 | pLexM-HsPQLC1(20-271)-FLAG | Expression of C-terminally FLAG-Tagged, N-Terminally Truncated (?1-19) HsPQLC1 in Mammalian Cells |
| 250418 | pLexM-HsPQLC1(24-271)-FLAG | Expression of C-terminally FLAG-Tagged, N-Terminally Truncated (?1-23) HsPQLC1 in Mammalian Cells |
| 250419 | pLexM-HsPQLC1(16-271)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-15), HsPQLC1 in Mammalian Cells |
| 250420 | pLexM-HsPQLC1(20-271)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-19), HsPQLC1 in Mammalian Cells |
| 250421 | pLexM-HsPQLC1(24-271)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-23), HsPQLC1 in Mammalian Cells |
| 250422 | pLexM-HsPQLC1(Δ100-125/GGGS)-FLAG | Expression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker) in Mammalian Cells |
| 250423 | pLexM-HsPQLC1(Δ100-125/GGGS/Δ257-271)-FLAG | Expression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker; Δ257-271) in Mammalian Cells |
| 250424 | pLexM-HsPQLC1(Δ100-125/3xGGGS)-FLAG | Expression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a 3xGGGS Linker) in Mammalian Cells |
| 250425 | pLexM-HsPQLC1(Δ100-125/3xGGGS/Δ257-271)-FLAG | Expression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by 3xGGGS Linker; Δ257-271) in Mammalian Cells |
| 250426 | pLexM-HsPQLC1(Δ100-125/GGGS)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker) in Mammalian Cells |
| 250427 | pLexM-HsPQLC1(Δ100-125/GGGS/Δ257-271)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker; Δ257-271) in Mammalian Cells |
| 250428 | pLexM-HsPQLC1(Δ100-125/3xGGGS)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a 3xGGGS Linker) in Mammalian Cells |
| 250429 | pLexM-HsPQLC1(Δ100-125/3xGGGS/Δ257-271)-TEV-GFP-His8 | Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by 3xGGGS Linker; Δ257-271) in Mammalian Cells |
| 250430 | pLexM-MmKDELR3-FLAG | Expression of C-terminally FLAG-Tagged Mouse KDELR3 in Mammalian Cells |