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CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.

Samelson AJ, Ariqat N, McKetney J, Rohanitazangi G, Parra Bravo C, Bose RS, Travaglini KJ, Lam VL, Goodness D, Ta T, Dixon G, Marzette E, Jin J, Tian R, Tse E, Abskharon R, Pan HS, Carroll EC, Lawrence RE, Gestwicki JE, Rexach JE, Eisenberg DS, Kanaan NM, Southworth DR, Gross JD, Gan L, Swaney DL, Kampmann M
Cell. 2026 Jan 28:S0092-8674(25)01487-4. doi: 10.1016/j.cell.2025.12.038. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
250736pAJS1197 Cul5-3xFlag-T2A-mAppleVector for lentiviral expression of Cul5-3x-Flag-T2A-mApple
250737pAJS1203 GFP-3xFlag-T2A-mAppleVector for lentiviral expression of EmGFP-3x-Flag-T2A-mApple
250738pAJS1205 GFP-Tau-1-50-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau1_50-T2A-mApple
250739pAJS1206 GFP-Tau-40-90-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau40-90-T2A-mApple
250740pAJS1207 GFP-Tau-80-130-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau80_130-T2A-mApple
250741pAJS1208 GFP-Tau-120-170-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau120_170-T2A-mApple
250742pAJS1209 GFP-Tau-160-210-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau160_210-T2A-mApple
250743pAJS1210 GFP-Tau-200-250-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau200_250-T2A-mApple
250744pAJS1211 GFP-Tau-240-290-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau240_290-T2A-mApple
250745pAJS1212 GFP-Tau-280-352-T2A-mAppleVector for lentiviral expression of GFP-0N3R-Tau80_130-T2A-mApple
250747pAJS1184 GFP-Tau-T2A-mAppleVector for expression of emGFP-0N3R-Tau-T2A-mApple. Ratiometric reporter for 0N3R tau levels
250748pAJS1154 GFPVector for lentiviral expression of GFP in iPSC-derived neurons. Control for pAJS1149
250751pAJS1228 His-Sumo-Tau-1-172Vector for bacterial expression of tau 1-172 with scarless SMNT3/Ulp1 cleavage
250752pAJS1229 His-Sumo-Tau-1-176Vector for bacterial expression of tau 1-176 with scarless SMNT3/Ulp1 cleavage
250753pAJS1200 SOCS4-3xFlag-T2A-mAppleVector for lentiviral expression of SOCS4-3x-Flag-T2A-mApple
250754pAJS1216 GFP-Tau-80-130-IRES-mAppleVector for lentiviral expression of GFP-0N3R-Tau80_130-IRES-mApple
250755pAJS1219 GFP-IRES-mAppleVector for lentiviral expression of GFP-IRES-mApple. Used as a control with plasmids pAJS1184, pAJS1216
250756pAJS1222 SOCS4-HA-IRES-Puro-T2A-mTagBFP2Vector for lentiviral expression of SOCS4-HA with a Puro-T2A-mTAGBFP2 marker
250758pAJS1085 pET His-Sumo-ORFVector for bacterial expression of proteins with scarless SMNT3/Ulp1 cleavage

Antibodies from Article