A bulk cell heterozygous knock-in strategy for targeted protein degradation
Liu B, Qi C, Kanie T
bioRxiv 2026.05.19.726384
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251478 | pUC19-Blast-P2A-dTAG | This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation. |
| 251479 | pUC19-Blast-P2A-IKZF3d | This vector serves as a PCR template to generate donor DNA that is used for knocking-in IKZF3d for conditional protein degradation. |
| 251480 | pUC19-Blast-P2A-HaloTag | This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation. |
| 251481 | pENTR221-miniCMVp-Blast-P2A-dTAG | This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation. MiniCMV promoter may enhance the expression of dTAG-tagged protein. |
| 251482 | pENTR221-miniCMVp-Blast-P2A-IKZF3d | This vector serves as a PCR template to generate donor DNA that is used for knocking-in IKZF3d for conditional protein degradation. MiniCMV promoter may enhance the expression of IKZF3d-tagged protein |
| 251483 | pENTR221-miniCMVp-Blast-P2A-HaloTag | This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation. MiniCMV promoter may enhance the expression of Halo-tagged protein. |
| 251484 | pENTR221-sEFp-Blast-P2A-dTAG | This vector serves as a PCR template to generate donor DNA that is used for knocking-in dTAG for conditional protein degradation. Short EF promoter enhances the expression of dTAG-tagged protein. |
| 251485 | pENTR221-sEFp-Blast-P2A-IKZF3d | This vector serves as a PCR template to generate donor DNA that is used for knocking-in IKZF3d for conditional protein degradation. Short EF promoter enhances the expression of IKZF3d-tagged protein. |
| 251486 | pENTR221-sEFp-Blast-P2A-HaloTag | This vector serves as a PCR template to generate donor DNA that is used for knocking-in HaloTag for conditional protein degradation. Short EF promoter enhances the expression of Halo-tagged protein. |